{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,11,15]],"date-time":"2023-11-15T11:17:18Z","timestamp":1700047038590},"reference-count":31,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2005,12,14]],"date-time":"2005-12-14T00:00:00Z","timestamp":1134518400000},"content-version":"vor","delay-in-days":3757,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[1995,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The polymerase chain reaction (PCR) was used to amplify a 149 base\u2010pair region of the cytomegalovirus (CMV) genome and a 551 base\u2010pair region of the HIV\u20101 proviral long terminal repeat (LTR) present in DNA extracted from post\u2010mor\u2010tem tissue. Multiple tissues (n=116) obtained from 16 patients which were subjected to PCR were also subjected to cell culture and histo\u2010pathological analyses. One hundred and seven samples (92%) contained CMV DNA and 66\/116 (57%) contained HIV proviral DNA at a level of \u226510 genomes. Both viruses were detected in 60\/116 (51.7%) of samples, with co\u2010infection most frequent in the lung (69%). Cell culture for CMV detected 9.3% of the PCR\u2010positive samples, whilst histology identified CMV inclusions in 15.9% of samples, all of which were CMV PCR\u2010positive. CMV was most frequently detected in adrenal and lung tissues by histology. These results show that co\u2010infection with CMV and HIV is a common occurrence in organs from AIDS patients and provide further evidence for a role of cytomegalovirus in the pathogenesis of AIDS. \u00a9 1995 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jmv.1890470106","type":"journal-article","created":{"date-parts":[[2007,3,3]],"date-time":"2007-03-03T02:40:43Z","timestamp":1172889643000},"page":"23-28","source":"Crossref","is-referenced-by-count":10,"title":["Use of the polymerase chain reaction to detect genomes of human immunodeficiency virus and cytomegalovirus in post\u2010mortem tissues"],"prefix":"10.1002","volume":"47","author":[{"given":"A.","family":"Webster","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J. E.","family":"McLaughlin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M. 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