{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,24]],"date-time":"2026-10-24T00:42:32Z","timestamp":1792802552138,"version":"4.5.0"},"reference-count":51,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2005,12,7]],"date-time":"2005-12-07T00:00:00Z","timestamp":1133913600000},"content-version":"vor","delay-in-days":3720,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[1995,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Enterovirus71 H (E71 H), an isolate from an adult patient with hand\u2010foot\u2010mouth disease (HFMD) in China, was serologically similar to the prototype strain E71 BrCr, which was isolated from a patient with aseptic meningitis. The study further analyzed the similarity of E71 H to E71 BrCr at the 5\u2032\u2010noncoding region (NCR), a location in genomic RNA that recently was found to be related to neurovirulence in poliovirus and Venezuelan equine encephalitis virus. Using a reverse transcription\u2010polymerase chain reaction (RT\u2010PCR) technique and a unique primer pair I, a 397 bp product was detected from E7 I BrCr, Cox A9 (Griggs), Cox A16 (NIH), Cox B1 (HA antigen 201\u20134681, Cox 65 (wild type), and ECHO 11 (Gregory), but <jats:italic>not<\/jats:italic> from E71 H, Cox A24 (Joseph), and ECHO 5 (Noyce). However, all of the viruses generated a 154 bp product using a universal enterovirus primer pair II. Further comparative analysis using primer\u2010directed sequencing of both the E71 H and E71 BrCr 154 bp products revealed that they differed by 12 bases. The variations between the two viruses were clustered in two loci, one in the region of nucleotides 43\u201361 with eight variations, and the other in the region of nucleotides 120\u2013133 with three variations. The differences within the 5\u2032\u2010NCR between the E71 H (HFMD) and the E71 BrCr (aseptic meningitis) viruses might provide a clue to explain why E71 was associated with two different clinical patterns: polio\u2010like disease in the United States, Australia, and Eastern Europe, HFMD in China, Japan, and Singapore. \u00a9 1995 Wiiey\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jmv.1890470209","type":"journal-article","created":{"date-parts":[[2007,3,3]],"date-time":"2007-03-03T02:25:40Z","timestamp":1172888740000},"page":"161-167","source":"Crossref","is-referenced-by-count":44,"title":["Enterovirus 71 isolated from China is serologically similar to the prototype E71 BrCr strain but differs in the 5\u2032\u2010noncoding 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