{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,12]],"date-time":"2026-03-12T04:07:10Z","timestamp":1773288430842,"version":"3.50.1"},"reference-count":14,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2005,12,7]],"date-time":"2005-12-07T00:00:00Z","timestamp":1133913600000},"content-version":"vor","delay-in-days":3659,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[1995,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The isolation and identification of the 87A strain of hepatitis E virus (HEV) by means of cell culture have been described previously. This paper reports the nucleotide sequence of a portion of this HEV strain. The RNA extracted from the supernatants of the different passages of the 87A strain cultured in the A549 cell line was reverse\u2010transcribed (RT) to cDNA, and then the polymerase chain reaction (PCR) amplification was carried out using the primers of HEV ET1.l region. The PCR products from 1) the supernatant of the infected cells at the fourth passage, 2) the virus concentrated by polyethylene glycol (PEG) precipitation at the tenth passage, and 3) the virus purified by a sucrose gradient at the tenth pas sage were sequenced. In addition, three other PCR products obtained from sera of acute hepa titis E patients in Beijing (B\u20109) and Guangzhou (G\u20109 and G\u201020) were also sequenced. The nucle otide sequences of the above four strains of HEV (located in the genome from positions 4545\u20134754) were compared to those of some reported HEV strains. The nucleotide sequences of the B\u20109 strain and the 87A strain were similar to the Burmese strain and may belong to the same branch of HEV. The nucleotide sequences of the G\u20109 strain and the G\u201020 strain were a novel and unique branch. The Chinese HEV strains are multiplex and variable in gene structure. \u00a9 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jmv.1890470403","type":"journal-article","created":{"date-parts":[[2007,3,3]],"date-time":"2007-03-03T04:16:35Z","timestamp":1172895395000},"page":"303-308","source":"Crossref","is-referenced-by-count":56,"title":["II. Existing variations on the gene structure of hepatitis E virus strains from some regions of China"],"prefix":"10.1002","volume":"47","author":[{"given":"Rutong","family":"Huang","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Naoki","family":"Nakazono","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Keizo","family":"Ishii","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Osamu","family":"Kawamata","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ryuji","family":"Kawaguchi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yutaka","family":"Tsukada","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,12,7]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1348-0421.1992.tb02061.x"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/0168-1702(93)90024-H"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1093\/oxfordjournals.bmb.a072409"},{"issue":"20","key":"e_1_2_1_5_1","doi-asserted-by":"crossref","first-page":"6689","DOI":"10.1128\/jb.175.20.6689-6694.1993","article-title":"Evolutionary relationships among magnetospirillurn strains inferred from phylogenetic analysis of 16s rDNA sequences","volume":"175","author":"Burgess JG","year":"1993","journal-title":"Journal of Bacteriology"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/0042-6822(92)90230-M"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1099\/0022-1317-73-5-1143"},{"issue":"2","key":"e_1_2_1_8_1","first-page":"284","article-title":"PCR and nucleic acid hybridization of a virus isolated from the patient's feces with hepatitis E in China","volume":"13","author":"Huang RT","year":"1992","journal-title":"Chinese Journal of Epidemiology"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.1890470402"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.1890370405"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/0002-9343(80)90200-4"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.2107574"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/0042-6822(91)90760-9"},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.89.2.559"},{"key":"e_1_2_1_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.1890410311"}],"container-title":["Journal of Medical Virology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjmv.1890470403","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jmv.1890470403","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,26]],"date-time":"2023-10-26T07:59:40Z","timestamp":1698307180000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jmv.1890470403"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1995,12]]},"references-count":14,"journal-issue":{"issue":"4","published-print":{"date-parts":[[1995,12]]}},"alternative-id":["10.1002\/jmv.1890470403"],"URL":"https:\/\/doi.org\/10.1002\/jmv.1890470403","archive":["Portico"],"relation":{},"ISSN":["0146-6615","1096-9071"],"issn-type":[{"value":"0146-6615","type":"print"},{"value":"1096-9071","type":"electronic"}],"subject":[],"published":{"date-parts":[[1995,12]]}}}