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The RT\u2010PCR product size was\u2010500 bp (479 bp for Poliovirus, 500 bp for Cox\u2010sackievirus) and was visualized using ethidium bromide\u2010stained gels. Assay 1 utilized Moloney Murine Leukaemia Virus Reverse Transcriptase (MMLV\u2010RTase) for reverse transcription and Taq polymerase for subsequent PCR. Assay 2 utilized a thermoactive DNA polymerase of <jats:italic>Thermus thermophilus<\/jats:italic> (rTth enzyme) for both reverse transcription and DNA amplification. In addition, in Assay 2 reverse transcription and PCR wer eaccomplished within the same reaction tube. Both assays detected between 1 and 0.02 TCID<jats:sub>50<\/jats:sub> of prototype strains of Polio and Coxsackie type B viruses propagated in VERO cell and spiked in a pooled preparation of CSF samples from patients with noninfective neurological disorders. However, Assay 1 was 10\u2010fold more sensitive than Assay 2 when applied to the detection of enteroviral RNA in CSF samples from patients with etiologically well characterized acute aseptic meningitis. \u00a9 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jmv.1890470414","type":"journal-article","created":{"date-parts":[[2007,3,3]],"date-time":"2007-03-03T04:16:35Z","timestamp":1172895395000},"page":"378-385","source":"Crossref","is-referenced-by-count":23,"title":["Two different PCR assays to detect enteroviral RNA in CSF samples from patients with acute aseptic meningitis"],"prefix":"10.1002","volume":"47","author":[{"given":"Inmaculada","family":"Casas","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paul E.","family":"Klapper","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Graham 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