{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,4]],"date-time":"2025-11-04T15:36:04Z","timestamp":1762270564565},"reference-count":26,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2004,1,9]],"date-time":"2004-01-09T00:00:00Z","timestamp":1073606400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2004,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Human polyomaviruses JCV and BKV can cause several clinical manifestations in immunocompromised hosts, including progressive multifocal leukoencephalopathy (PML) and haemorrhagic cystitis. Molecular detection by polymerase chain reaction (PCR) is recognised as a sensitive and specific method for detecting human polyomaviruses in clinical samples. In this study, we developed a PCR assay using a single primer pair to amplify a segment of the <jats:italic>VP1<\/jats:italic> gene of JCV and BKV. An enzyme linked amplicon hybridisation assay (ELAHA) using species\u2010specific biotinylated oligonucleotide probes was used to differentiate between JCV and BKV. This assay (VP1\u2010PCR\u2010ELAHA) was evaluated and compared to a PCR assay targeting the human polyomavirus T antigen gene (<jats:italic>pol<\/jats:italic>\u2010<jats:italic>PCR<\/jats:italic>). DNA sequencing was used to confirm the polyomavirus species identified by the VP1\u2010PCR\u2010ELAHA and to determine the subtype of each JCV isolate. A total of 297 urine specimens were tested and human polyomavirus was detected in 105 specimens (35.4%) by both PCR assays. The differentiation of JCV and BKV by the VP1\u2010PCR\u2010ELAHA showed good agreement with the results of DNA sequencing. Further, DNA sequencing of the JCV positive specimens showed the most prevalent JCV subtype in our cohort was 2a (27%) followed by 1b (20%), 1a (15%), 2c (14%), 4 (14%) and 2b (10%). The results of this study show that the VP1\u2010PCR\u2010ELAHA is a sensitive, specific and rapid method for detecting and differentiating human polyomaviruses JC and BK and is highly suitable for routine use in the clinical laboratory. J. Med. Virol. 72:467\u2013472, 2004. \u00a9 2004 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jmv.20005","type":"journal-article","created":{"date-parts":[[2004,1,23]],"date-time":"2004-01-23T12:02:29Z","timestamp":1074859349000},"page":"467-472","source":"Crossref","is-referenced-by-count":14,"title":["Simultaneous detection and differentiation of human polyomaviruses JC and BK by a rapid and sensitive PCR\u2010ELAHA assay and a survey of the JCV subtypes within an Australian population"],"prefix":"10.1002","volume":"72","author":[{"given":"David M.","family":"Whiley","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Katherine E.","family":"Arden","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ian M.","family":"Mackay","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Melanie 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