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In this study, two sets of virus variants including T1P1\u2010L4\/T1P1\u2010S1 and CJN\u2010L1\/CJN\u2010S1 derived from two strains (T1P1 and CJN) of the JE virus were used to evaluate the effects of genomic variations on virus entry. Each set of virus variant (T1P1\u2010L4\/T1P1\u2010S1 or CJN\u2010L1\/CJN\u2010S1) possessed a single amino acid variation in the E protein. The variation of Glu\/Lys at E\u2010306 was found between T1P1\u2010L4 and T1P1\u2010S1 whereas the same variation at E\u2010138 was seen between CJN\u2010L1 and CJN\u2010S1. The results showed that heparan sulfate (HS) differentially expressed on the surface of different types of host cells was essential for JE virus infection as shown in an evident difference in attachment efficiency between CHO\u2010K1 cells and its mutant with defects in GAG biosynthesis. Furthermore, differential interaction of heparin with the envelope protein of JE virus variants implies the significance of virus mutations (especially Lys for E\u2010138 and\/or E306 in this case) that are rather likely involved in determining efficiencies of viral attachment, penetration, and eventual infection. J. Med. Virol. 72:618\u2013624, 2004. \u00a9 2004 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jmv.20025","type":"journal-article","created":{"date-parts":[[2004,2,19]],"date-time":"2004-02-19T10:00:24Z","timestamp":1077184824000},"page":"618-624","source":"Crossref","is-referenced-by-count":47,"title":["Differential binding efficiency between the envelope protein of Japanese encephalitis virus variants and heparan sulfate on the cell 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