{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,27]],"date-time":"2026-06-27T19:18:36Z","timestamp":1782587916163,"version":"3.54.5"},"reference-count":38,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2004,3,18]],"date-time":"2004-03-18T00:00:00Z","timestamp":1079568000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2004,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Aseptic meningitis is a frequent diagnosis in emergency departments. Nevertheless, viral investigations are not carried out currently and the viral etiology in adult population has not been studied extensively. We conducted a prospective study including all consecutive patients undergoing lumbar puncture during a 15 months period in an adult emergency department. Bloody and purulent cerebrospinal fluid (CSF) were excluded. The main tests undertaken were: CSF genomic amplification by the polymerase chain reaction (PCR) for neurotropic viruses and serum and CSF interferon\u2010alpha (IFN\u2010\u03b1) measurements. Among 194 patients included, 45 had and 149 did not have aseptic meningitis. Of 45 patients with aseptic meningitis, 10 had alternative non\u2010virological final diagnosis, and 35\/45 were presumed to have neurological disorders of viral origin. Patients (27\/35) completed virological analysis: 21\/27 (78%) had either positive viral PCR (enterovirus: 8 patients, <jats:italic>Varicella zoster<\/jats:italic> virus (VZV): 5, Epstein\u2010Barr virus (EBV): 2, herpes simplex virus (HSV): 1, human herpes virus 6: 1) or only raised serum or CSF IFN\u2010\u03b1 (4 patients). Overall, 59% of patients with a positive viral PCR had either CSF or serum raised IFN\u2010\u03b1. Twentyone patients without meningitis had either positive viral PCR (enterovirus: 3 patients) or only high serum IFN\u2010\u03b1 level (18 patients). In the setting of aseptic meningitis diagnosed in an adult emergency department, viruses are the most common agents encountered, with enterovirus and VZV as the two main etiological agents. Current CSF viral genome amplification and IFN\u2010\u03b1 measurement are informative and could be useful to confirm the viral origin of various neurological disorders, although the sensitivity and specificity of IFN\u2010\u03b1 measurement for the diagnosis of viral infection need further confirmation. J. Med. Virol. 73:137\u2013146, 2004. \u00a9 2004 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jmv.20068","type":"journal-article","created":{"date-parts":[[2004,3,19]],"date-time":"2004-03-19T15:54:20Z","timestamp":1079711660000},"page":"137-146","source":"Crossref","is-referenced-by-count":25,"title":["Prevalence of viral infection markers by polymerase chain reaction amplification and interferon\u2010alpha measurements among patients undergoing lumbar puncture in an emergency 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