{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,26]],"date-time":"2026-10-26T18:02:03Z","timestamp":1793037723056,"version":"4.5.0"},"reference-count":37,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2004,7,13]],"date-time":"2004-07-13T00:00:00Z","timestamp":1089676800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100005972","name":"Deutsche Krebshilfe","doi-asserted-by":"crossref","award":["70-2623-So I"],"award-info":[{"award-number":["70-2623-So I"]}],"id":[{"id":"10.13039\/501100005972","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2004,9]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>The oncogenic potential of the high\u2010risk human papillomavirus (HPV) genotypes (types 16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 66 and 68) depends on the expression of the two viral oncogenes E6 and E7. Thus, the detection of HPV E6\/E7 oncogene transcripts could serve as a factor in the evaluation of a risk of development of cervical intraepithelial neoplasia (CIN) and its progression to cervical cancer. A nested RT\u2010PCR assay for the detection of E6\/E7 oncogene transcripts of all known high\u2010risk HPV genotypes was established. In the study described, 779 high\u2010risk HPV\u2010DNA\u2010positive cervical scrapes exhibiting all grades of CIN, including non\u2010dysplastic cervical mucosa (CIN 0), were examined. Spliced E6\/E7 oncogene transcripts of all the high\u2010risk HPVs were detected in numerous samples, with an overall detection rate of 47%. In 227 cases with agreement between the cytologic and histologic findings, the prevalence increased with lesion severity: CIN 0, 18%; CIN I, 58%; CIN II, 77%; CIN III, 84%. Multiple transcriptionally active high\u2010risk HPVs were detected in 12% (33\/279) of patients with multiple high\u2010risk HPV infections. This work sets the stage for a prospective follow\u2010up study currently being undertaken to evaluate the prognostic relevance of the detection of high\u2010risk HPV E6\/E7 oncogene transcripts for the persistence of a high risk HPV infection, and the possible evolution and further development of a CIN. Future applications of the assay described may include the monitoring of women in studies investigating antiviral treatment or vaccination. J. Med. Virol. 74:107\u2013116, 2004. \u00a9 2004 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jmv.20153","type":"journal-article","created":{"date-parts":[[2004,7,13]],"date-time":"2004-07-13T10:04:25Z","timestamp":1089713065000},"page":"107-116","source":"Crossref","is-referenced-by-count":109,"title":["Detection of high\u2010risk human papillomavirus E6 and E7 oncogene transcripts in cervical scrapes by nested RT\u2010polymerase chain reaction"],"prefix":"10.1002","volume":"74","author":[{"given":"Karl","family":"Sotlar","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Annette","family":"Stubner","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Diemer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Susanne","family":"Menton","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"Menton","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Klaus","family":"Dietz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Diethelm","family":"Wallwiener","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Reinhard","family":"Kandolf","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Burkhard","family":"B\u00fcltmann","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2004,7,13]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1093\/jnci\/87.11.796"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(87)90021-2"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.58.3.991-995.1986"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1099\/0022-1317-71-5-1243"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/0959-8049(95)00650-8"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.2910560206"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.2537532"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1006\/gyno.1995.1213"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.38.1.357-361.2000"},{"key":"e_1_2_1_11_1","doi-asserted-by":"crossref","first-page":"3905","DOI":"10.1002\/j.1460-2075.1989.tb08570.x","article-title":"HPV16 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