{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,8]],"date-time":"2025-12-08T22:06:07Z","timestamp":1765231567230},"reference-count":31,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2004,8,20]],"date-time":"2004-08-20T00:00:00Z","timestamp":1092960000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2004,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Dengue (DEN) is an acute mosquito borne viral disease of mankind. Off late it has become an important public health concern in Southeast Asia. Although, all the four known dengue virus serotypes (DEN\u20101 to 4) are reported from time to time, in the recent past, DEN\u20102 has emerged as the predominant type, being the causative agent of several outbreaks of dengue fever (DF) and dengue haemorrhagic fever (DHF) in India. To elucidate the true molecular epidemiology of these viruses, we have sequenced C\u2010prM gene junction (454 nucleotides) of 11 DEN\u20102 viruses directly from patient serum. The C\u2010prM gene junction was amplified initially by reverse transcription\u2010polymerase chain reaction followed by automated DNA sequencing. These sequences provide unique information with regard to molecular epidemiology when compared to other DEN\u20102 sequences from diverse geographic origins. The sequence analysis revealed that most of the mutations in this region remained silent, except a few at the carboxy\u2010terminal of the capsid. Reported phylogenetic analysis classifies DEN\u20102 viruses into five distinct genotypes. The Gwalior DEN\u20102 viruses, included in the present study were classified into genotype\u2010IV, and were found to be most closely related to Delhi 1996 DEN\u20102 viruses and FJ 10\/11 strains prevalent in the Fujian state of China. However, two earlier Indian isolates of DEN\u20102 were classified into genotype\u2010V. The present study indicates that genotype V of DEN\u20102 has been replaced by genotype IV during the past decade, which continues to circulate silently in north India, and have the potential to reemerge and cause major epidemics of DF and DHF. J. Med. Virol. 74:314\u2013322, 2004. \u00a9 2004 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jmv.20166","type":"journal-article","created":{"date-parts":[[2004,8,23]],"date-time":"2004-08-23T13:22:40Z","timestamp":1093267360000},"page":"314-322","source":"Crossref","is-referenced-by-count":52,"title":["Emergence and continued circulation of dengue\u20102 (genotype IV) virus strains in northern India"],"prefix":"10.1002","volume":"74","author":[{"given":"Paban Kumar","family":"Dash","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Man 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