{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T09:38:03Z","timestamp":1791020283620,"version":"4.1.0"},"reference-count":26,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2004,10,13]],"date-time":"2004-10-13T00:00:00Z","timestamp":1097625600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2004,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The detection of the enterovirus genome in cerebrospinal fluid (CSF) by PCR techniques has proved to be more sensitive than traditional cell culture for the diagnosis of enterovirus meningitis. However, PCR assays are time consuming and labor intensive, particularly if separate hybridization steps are used to confirm the specificity of positive findings. The aim of this study was to develop a one\u2010step real\u2010time RT\u2010PCR assay with LightCycler (LC) technology that was sensitive, rapid, and easy to perform in routine practice. The enterovirus detection limit was determined by testing 10\u2010fold limiting dilution series of cell culture stocks with the echovirus 25 (E\u201025) prototype strain and with the third European Union Quality Control Concerted Action (EU\u2010QCCA) enterovirus proficiency panel. A total of 100 CSF specimens were investigated in a comparative study. With the E\u201025 strain, the detection limit of the real\u2010time assay was 286 TCID<jats:sub>50<\/jats:sub>\/ml (50% tissue culture infective dose). When samples of the EU\u2010QCCA panel were tested, our assay gave identical results (detection limit down to 3.6 TCID<jats:sub>50<\/jats:sub>\/ml) to those of the reference laboratory, which used one\u2010step RT\u2010PCR assay. When CSF specimens were tested, there was a correlation between the real\u2010time assay and the conventional in\u2010house assay in 96 of 100 CSFs tested. This one\u2010step real\u2010time assay allows rapid enterovirus detection in CSF since results are obtained in 3 hr as against 36 hr with the \u201cin\u2010house\u201d RT\u2010PCR assay. This new assay is now being used in routine practice, and allows diagnosis on a daily basis. J. Med. Virol. 74:604\u2013611, 2004. \u00a9 2004 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jmv.20217","type":"journal-article","created":{"date-parts":[[2004,10,13]],"date-time":"2004-10-13T17:38:21Z","timestamp":1097689101000},"page":"604-611","source":"Crossref","is-referenced-by-count":23,"title":["Improved diagnosis on a daily basis of enterovirus meningitis using a one\u2010step real\u2010time RT\u2010PCR assay"],"prefix":"10.1002","volume":"74","author":[{"given":"Christine","family":"Archimbaud","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Audrey","family":"Mirand","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martine","family":"Chambon","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christel","family":"Regagnon","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean\u2010Luc","family":"Bailly","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H\u00e9l\u00e8ne","family":"Peigue\u2010Lafeuille","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C\u00e9cile","family":"Henquell","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2004,10,13]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"crossref","first-page":"589","DOI":"10.1128\/JCM.36.2.589-591.1998","article-title":"Comparison of use of cerebrospinal fluid, serum, and throat swab specimens in diagnosis of enteroviral acute neurological infection by a rapid RNA detection PCR assay","volume":"36","author":"Andreoletti L","year":"1998","journal-title":"J Clin Microbiol"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.41.10.4605-4610.2003"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/0166-0934(94)00173-E"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.2039"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.64.2.669-677.1998"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.10138"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1386-6532(00)00176-1"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0166-0934(01)00300-7"},{"key":"e_1_2_1_10_1","doi-asserted-by":"crossref","first-page":"309","DOI":"10.4049\/jimmunol.84.3.309","article-title":"Typing of viruses by combinations of antiserum pools. 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