{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T00:43:28Z","timestamp":1791333808382,"version":"4.3.1"},"reference-count":30,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2004,10,13]],"date-time":"2004-10-13T00:00:00Z","timestamp":1097625600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Estonian Foundation","award":["4739"],"award-info":[{"award-number":["4739"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2004,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Tick\u2010borne encephalitis virus (TBEV) is a severe problem in Estonia. In the present article the first genetic analysis of Estonian TBEV strains is described. In total, seven TBEV strains were isolated from ticks (<jats:italic>Ixodes ricinus<\/jats:italic> and <jats:italic>I. persulcaus<\/jats:italic>), rodents (<jats:italic>Apodemus agrarius<\/jats:italic> and <jats:italic>Cletrionomys glareolus<\/jats:italic>), and serum from a tick\u2010borne encephalitis (TBE) patient. The nucleic acid sequences of the viral genome encoding almost the complete E protein (nt 41\u20131250) and the 3\u2032\u2010NCR\u2010termini of the Estonian TBEV strains were determined by direct sequencing of RT\u2010PCR products. The results showed that all three known TBEV subtypes, Western TBEV (W\u2010TBEV), Far\u2010Eastern TBEV (FE\u2010TBEV), and Siberian TBEV (S\u2010TBEV), co\u2010circulate in Estonia. The Estonian TBEV strains of the S\u2010TBEV and W\u2010TBEV subtypes clustered with the previously reported strains from Latvia and Lithuania. Within the FE\u2010TBEV subtype, however, the Estonian strain Est2546 clustered together with the strain Sofjin, originating from the Far\u2010East of Russia, but not with the strain RK1424, isolated in the neighboring Latvia. This suggests a different evolutionary history for the Estonian and the Latvian strains in the FE\u2010TBEV subtype. The Estonian TBEV strain (Est3535), which belonged to the S\u2010TBEV subtype, had an organization of the 3\u2032\u2010NCR similar to that of strains from the Far\u2010East of Russia (Irkutsk). The 3\u2032\u2010NCRs of Estonian strains of the W\u2010TBEV subtype (Est3051, Est3053, Est3476, and Est3509) were very similar to those of the strain Ljubljana I from the Balkans. In the 3\u2032\u2010NCR sequence of the Estonian strain Est2546, which belonged to the FE\u2010TBEV subtype, a deletion from position 10461 to 10810 extending approximately 10 nucleotides into the core element, was detected. J. Med. Virol. 74:580\u2013588, 2004. \u00a9 2004 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jmv.20224","type":"journal-article","created":{"date-parts":[[2004,10,13]],"date-time":"2004-10-13T17:38:17Z","timestamp":1097689097000},"page":"580-588","source":"Crossref","is-referenced-by-count":96,"title":["Characterization of tick\u2010borne encephalitis virus from Estonia"],"prefix":"10.1002","volume":"74","author":[{"given":"Irina","family":"Golovljova","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sirkka","family":"Vene","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katarina Brus","family":"Sj\u00f6lander","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Veera","family":"Vasilenko","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexander","family":"Plyusnin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"\u00c5ke","family":"Lundkvist","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2004,10,13]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.mi.44.100190.003245"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0934-8840(99)80013-9"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1086\/515195"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1099\/0022-1317-80-1-179"},{"key":"e_1_2_1_6_1","unstructured":"FelsensteinJ.1993.PHYLIP: Phylogeny inference package version 3.5c. 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