{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T16:52:12Z","timestamp":1791219132665,"version":"4.1.0"},"reference-count":22,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2004,11,12]],"date-time":"2004-11-12T00:00:00Z","timestamp":1100217600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2005,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Although most genes are highly conserved among strains of human cytomegalovirus (HCMV), several are unusually variable. By analyzing the sequence of two variable genes (<jats:italic>UL146<\/jats:italic> and <jats:italic>UL74<\/jats:italic>) amplified directly from whole blood DNA extracts, multiple HCMV strains were detected in blood samples from 5\/11 virus\u2010infected renal transplant patients. These five patients were seronegative prior to transplantation and their likely acquisition of the virus was via the donated organ; HCMV\u2010positive immunocompetent donors may thus be capable of harboring and transmitting multiple virus genotypes. In sequential samples taken up to 140 days post transplant no mutations in either gene were detected from 9\/10 patients, and in the remaining patient a single nucleotide change was detected in <jats:italic>UL146<\/jats:italic>, and none in <jats:italic>UL74<\/jats:italic>. All sequences grouped into previously defined genotypes, with the detection of multiple members of the <jats:italic>UL74<\/jats:italic> group 5 genotype establishing this previously tentative genotype. Additionally, identical sequences were identified in viruses from different patients, including examples from geographically distinct regions. Thus, although <jats:italic>UL146<\/jats:italic> and <jats:italic>UL74<\/jats:italic> exhibit impressive variation among strains, their sequences are maintained stably within and between infected individuals, suggesting that sequence differences between genotypes may be driven by differing gene function. J. Med. Virol. 75:42\u201346, 2005. \u00a9 2005 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jmv.20235","type":"journal-article","created":{"date-parts":[[2004,11,12]],"date-time":"2004-11-12T22:51:11Z","timestamp":1100299871000},"page":"42-46","source":"Crossref","is-referenced-by-count":63,"title":["Stability of human cytomegalovirus genotypes in persistently infected renal transplant recipients"],"prefix":"10.1002","volume":"75","author":[{"given":"Richard","family":"Stanton","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Diana","family":"Westmoreland","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julie D.","family":"Fox","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrew J.","family":"Davison","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gavin W.G.","family":"Wilkinson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2004,11,12]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1099\/vir.0.18952-0"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1096-9071(200005)61:1<138::AID-JMV22>3.0.CO;2-#"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1086\/344238"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.immunol.15.1.675"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/s007050050410"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM200105033441804"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.10383"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1006\/viro.1994.1044"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1099\/vir.0.79888-0"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(00)02350-3"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.72.10.8191-8197.1998"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.virol.2003.09.036"},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.1086\/515262"},{"key":"e_1_2_1_15_1","first-page":"391","article-title":"Interleukin\u20108: An expanding universe beyond neutrophil chemotaxis and activation","volume":"72","author":"Mukaida N","year":"2000","journal-title":"Int J Hematol"},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.1006\/viro.2001.1274"},{"key":"e_1_2_1_17_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.96.17.9839"},{"key":"e_1_2_1_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/rmv.315"},{"key":"e_1_2_1_19_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.76.21.10841-10848.2002"},{"key":"e_1_2_1_20_1","doi-asserted-by":"publisher","DOI":"10.1086\/367900"},{"key":"e_1_2_1_21_1","doi-asserted-by":"publisher","DOI":"10.1097\/00007890-200203270-00025"},{"key":"e_1_2_1_22_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.39.6.2219-2226.2001"},{"key":"e_1_2_1_23_1","doi-asserted-by":"crossref","first-page":"3662","DOI":"10.1128\/JCM.36.12.3662-3669.1998","article-title":"Sequence variation within three important cytomegalovirus gene regions in isolates from four different patient populations","volume":"36","author":"Zweygberg WB","year":"1998","journal-title":"J Clin Microbiol"}],"container-title":["Journal of Medical Virology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjmv.20235","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jmv.20235","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,17]],"date-time":"2023-11-17T05:18:54Z","timestamp":1700198334000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jmv.20235"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2004,11,12]]},"references-count":22,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2005,1]]}},"alternative-id":["10.1002\/jmv.20235"],"URL":"https:\/\/doi.org\/10.1002\/jmv.20235","archive":["Portico"],"relation":{},"ISSN":["0146-6615","1096-9071"],"issn-type":[{"value":"0146-6615","type":"print"},{"value":"1096-9071","type":"electronic"}],"subject":[],"published":{"date-parts":[[2004,11,12]]}}}