{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,22]],"date-time":"2026-06-22T10:31:16Z","timestamp":1782124276176,"version":"3.54.5"},"reference-count":28,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2005,2,15]],"date-time":"2005-02-15T00:00:00Z","timestamp":1108425600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2005,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>This study compares herpes simplex virus (HSV) type\u2010specific loop\u2010mediated isothermal amplification (LAMP) with virus isolation and real\u2010time PCR. Genital tract specimens were obtained from 25 patients with genital lesions; two swab samples were collected from the vulva and cervix of each patient, for a total of 50 specimens. After culturing, 10 of 50 (20%) samples were positive for HSV\u20101 and 12 of 50 (24%) samples were positive for HSV\u20102. None of the patients excreted both HSV\u20101 and HSV\u20102 virus. An original HSV type\u2010specific LAMP assay (30 min reaction) was compared with virus isolation and HSV type\u2010specific real\u2010time PCR. Viral DNA was detected by LAMP in 9 of 10 HSV\u20101 isolated samples and 11 of 12 HSV\u20102 isolated samples. No viral DNA was detected in samples without virus isolation. Thus, if virus isolation was used as the standard method, the LAMP protocol was highly sensitive and specific. In comparing LAMP to real\u2010time PCR, viral DNA was detected by the LAMP method in 9 of 12 HSV\u20101 DNA positive samples and 11 of 18 HSV\u20102 DNA positive samples. If real\u2010time PCR was used as the standard method, then, sensitivity of the LAMP method (in particular, for HSV\u20102) was low. Taking this into consideration, the LAMP reaction was extended to 60 min. This led to an increase in sensitivity, resulting in an additional one and three samples testing positive for HSV\u20101 LAMP and HSV\u20102 LAMP, respectively, compared to the original LAMP protocol. Therefore, the sensitivity of the LAMP method increased to about 80%. J. Med. Virol. 75:583\u2013587, 2005. \u00a9 2005 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jmv.20309","type":"journal-article","created":{"date-parts":[[2005,2,16]],"date-time":"2005-02-16T01:20:01Z","timestamp":1108516801000},"page":"583-587","source":"Crossref","is-referenced-by-count":28,"title":["Comparison of loop\u2010mediated isothermal amplification, real\u2010time PCR, and virus isolation for the detection of herpes simplex virus in genital 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