{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T14:49:54Z","timestamp":1786978194051,"version":"build-2736575974"},"reference-count":37,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2005,6,23]],"date-time":"2005-06-23T00:00:00Z","timestamp":1119484800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2005,8]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Genetically different subpopulations were identified and purified from Japanese Encephalitis virus (JEV). Those with small plaques (SPs; &lt;2 mm in diameter), derived from strains of T1P1, CJN, and CC27, were more competent than those with large plaques (LPs; &gt;5 mm in diameter) when passaged in Neuro\u20102a cells. Differences in amino acids between SPs and LPs from each strain were shown in the viral envelope (E) protein. The amino acid at E\u2010306 was Glu in LP but was substituted by Lys in SP in the T1P1 strain. A similar substitution occurred at E\u2010138 in the CJN strain. However, the amino acid was Asp in LP but was substituted by Asn in SP at E\u2010389 in the CC27 strain. All SPs were shown to have a higher affinity to the cellular membrane when compared to LPs, and this resulted in more\u2010efficient infection of Neuro\u20102a cells, suggesting that the differential fitness of JEV variants to Neuro\u20102a cells appeared in the early phase of infection. In addition, glycosaminoglycans (GAGs) on the surface of many mammalian cells have been demonstrated to be critical for infection by JEV, especially SP variants. The present results suggest that T1P1\u2010SP1 viruses infected Neuro\u20102a cells more efficiently in spite of the sparse distribution of cell surface GAGs. We conclude that highly sulfated forms of GAGs expressed by Neuro\u20102a cells play an important role in selecting JEV variants with specific mutations in the E glycoprotein. J. Med. Virol. 76:583\u2013592, 2005. \u00a9 2005 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jmv.20406","type":"journal-article","created":{"date-parts":[[2005,6,23]],"date-time":"2005-06-23T20:27:13Z","timestamp":1119558433000},"page":"583-592","source":"Crossref","is-referenced-by-count":35,"title":["Fitness of Japanese encephalitis virus to Neuro\u20102a cells is determined by interactions of the viral envelope protein with highly sulfated glycosaminoglycans on the cell 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