{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,14]],"date-time":"2026-09-14T12:30:30Z","timestamp":1789389030504,"version":"build-2803163510"},"reference-count":10,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2006,4,20]],"date-time":"2006-04-20T00:00:00Z","timestamp":1145491200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2006,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Three hundred eighteen European cows and 115 buffaloes were vaccinated with locally prepared Smithburn vaccine, of which, 100 cows and 20 buffaloes were pregnant. Twenty\u2010eight cows aborted within 72 days post\u2010vaccination, buffaloes did not abort. Blood samples collected 77 days post\u2010vaccination from aborted cows, 17 pregnant cows, 5 pregnant buffaloes, and 32 non\u2010pregnant cows. Sera were tested by ELISA for anti\u2010RVF IgM and IgG. All aborted cows were strongly positive for IgG. Five of 17 cows and two of five buffaloes that did not abort were IgG positive. The percentage of IgM positives in aborted cows was 25% and 0% in non\u2010aborted cows. The percentage of IgG positives in pregnant non\u2010aborted cows was lower than in non\u2010pregnant cows. The percentage of IgG positives of non\u2010pregnant cows was lower than pregnant aborted cows. Virus was isolated from one aborted fetus. The nucleotide sequence of fetus virus was compared to Smithburn of Onderstepoort, local Smithburn and virus isolates from 1993 to 1994 and 1977 RVF outbreaks. The nucleotide sequences of Onderstepoort and Egyptian Smithburn vaccines were almost identical. The sequences of 1993\u20131994 isolates were identical to 1977 outbreak virus. Virus from the fetus had two mutations; it is apparently a variant that is genetically distant from local Smithburn and Onderstepoort vaccines. Fetus virus was genetically distant from virus of 1993\/1994 and 1977 outbreaks. In conclusion, antibody response to vaccination with local Smithburn had occurred in some, but not all the cows and buffaloes. Virus isolation from the fetus suggests in utero transmission of used vaccine virus, which resulted in high abortions in European cows. J. Med. Virol. 78:787\u2013791, 2006. \u00a9 2006 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jmv.20624","type":"journal-article","created":{"date-parts":[[2006,4,20]],"date-time":"2006-04-20T15:51:14Z","timestamp":1145548274000},"page":"787-791","source":"Crossref","is-referenced-by-count":115,"title":["Adverse response of non\u2010indigenous cattle of European breeds to live attenuated Smithburn Rift Valley fever vaccine"],"prefix":"10.1002","volume":"78","author":[{"given":"Boulos","family":"Botros","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adel","family":"Omar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Khairat","family":"Elian","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gihan","family":"Mohamed","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Atef","family":"Soliman","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adel","family":"Salib","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Diaa","family":"Salman","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Magdi","family":"Saad","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kenneth","family":"Earhart","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2006,4,20]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0923-2516(89)80090-1"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/0140-6736(93)92128-G"},{"key":"e_1_2_1_4_1","first-page":"45","article-title":"An inactivated Rift Valley fever vaccine","volume":"48","author":"Barnard BJH","year":"1977","journal-title":"J S Afr Vet Assoc"},{"key":"e_1_2_1_5_1","first-page":"895","article-title":"Immunological response of Egyptian fat\u2010tail sheep to inactivated and live attenuated Smithburn Rift Valley fever vaccines","volume":"55","author":"Botros B","year":"1995","journal-title":"J Egypt Vet Med Assoc"},{"key":"e_1_2_1_6_1","first-page":"119","article-title":"Hydrops amnii in sheep associated with hydranencephaly and arthrogryposis with Wesselsbron disease and Rift Valley fever viruses as etiological agents","volume":"44","author":"Coetzer JA","year":"1977","journal-title":"Onderstepoort J Vet Res"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1093\/bib\/5.2.150"},{"key":"e_1_2_1_8_1","first-page":"1138","article-title":"Enzyme\u2010linked immunosorbent assay for detection of antibodies to Rift Valley fever virus in ovine and bovine sera","volume":"48","author":"Meegan JM","year":"1987","journal-title":"Am J Vet Res"},{"key":"e_1_2_1_9_1","first-page":"1042","article-title":"Pathogenicity and immunogenicity of a mutagen\u2010attenuated Rift Valley fever virus immunogen in pregnant ewes","volume":"48","author":"Morrill JC","year":"1987","journal-title":"Am J Vet Res"},{"key":"e_1_2_1_10_1","first-page":"1","article-title":"Rift Valley fever: The neurotropic adaptation of virus and experimental use of this modified virus as a vaccine","volume":"40","author":"Smithburn KC","year":"1949","journal-title":"B J Exper Pathol"},{"key":"e_1_2_1_11_1","first-page":"261","article-title":"The use of veterinary vaccines for prevention and control of Rift Valley fever: Memorandum from a WHO\/FAO meeting","volume":"61","author":"WHO","year":"1983","journal-title":"Bull WHO"}],"container-title":["Journal of Medical Virology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjmv.20624","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jmv.20624","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,15]],"date-time":"2023-11-15T14:54:23Z","timestamp":1700060063000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jmv.20624"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2006,4,20]]},"references-count":10,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2006,6]]}},"alternative-id":["10.1002\/jmv.20624"],"URL":"https:\/\/doi.org\/10.1002\/jmv.20624","archive":["Portico"],"relation":{},"ISSN":["0146-6615","1096-9071"],"issn-type":[{"value":"0146-6615","type":"print"},{"value":"1096-9071","type":"electronic"}],"subject":[],"published":{"date-parts":[[2006,4,20]]}}}