{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,21]],"date-time":"2025-10-21T14:54:58Z","timestamp":1761058498597},"reference-count":51,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2006,7,17]],"date-time":"2006-07-17T00:00:00Z","timestamp":1153094400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2006,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Recent studies indicate that a defective proliferative response of HIV\u2010specific CD8+ T cells is associated with the lack of virologic control in chronic HIV infection in humans. The possible mechanisms that might be responsible for the reduced proliferative potential of HIV\u2010specific CD8+ T cells and conditions conducive to the proliferation of CD8+ T cells were examined in 14 HIV\u2010infected individuals and 7 HIV\u2010uninfected controls using CFSE labeling and flow cytometry techniques, and analyzed data using 2 quantitative measurements: the percentages of proliferating CD8+ T cells (Tp), and the maximum number of cell divisions (Dm) after stimulation. It was found that CD8+ T cells from HIV\u2010infected and \u2010uninfected subjects proliferated equally well after polyclonal stimulation by phylohemagglutinin A (PHA); both groups reached a Tp of 92%\u201396% and a Dm of 5\u20138. However, in HIV\u2010infected subjects, proliferation of HIV\u2010 and CMV\u2010specific CD8+ T cells was significantly reduced compared to proliferation of CMV\u2010 specific CD8+ T cells from HIV\u2010uninfected subjects. These defective proliferative responses of HIV\u2010 and CMV\u2010specific CD8+ T cells were restored by the addition of IL\u20102 at the time of stimulation. These results may have implications for the design of immune modulation strategies in vivo. J. Med. Virol. 78:1147\u20131157, 2006. \u00a9 2006 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jmv.20675","type":"journal-article","created":{"date-parts":[[2006,7,18]],"date-time":"2006-07-18T04:24:08Z","timestamp":1153196648000},"page":"1147-1157","source":"Crossref","is-referenced-by-count":13,"title":["Interleukin\u20102 reconstitutes defective human immunodeficiency virus (HIV), and cytomegalovirus (CMV) specific CD8+ T cell proliferation in HIV 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