{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,3]],"date-time":"2026-04-03T16:12:00Z","timestamp":1775232720744,"version":"3.50.1"},"reference-count":33,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2006,8,22]],"date-time":"2006-08-22T00:00:00Z","timestamp":1156204800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2006,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>A microarray method was developed for simultaneous detection and identification of six species of <jats:italic>Orthopoxvirus<\/jats:italic> (OPV) including <jats:italic>Variola<\/jats:italic>, <jats:italic>Monkeypox<\/jats:italic>, <jats:italic>Cowpox<\/jats:italic>, <jats:italic>Camelpox<\/jats:italic>, <jats:italic>Vaccinia<\/jats:italic>, and <jats:italic>Ectromelia<\/jats:italic> viruses. The method allowed us to discriminate OPV species from varicella\u2010zoster virus (VZV), <jats:italic>Herpes Simplex<\/jats:italic> <jats:italic>1<\/jats:italic> virus (HSV\u20101), and <jats:italic>Herpes Simplex<\/jats:italic> <jats:italic>2<\/jats:italic> virus (HSV\u20102) that cause infections with clinical manifestations similar to OPV infections. The nucleotide sequences of the <jats:italic>C23L\/B29R<\/jats:italic> and the <jats:italic>B19R<\/jats:italic> genes identified for 86 and 72 different OPV strains, respectively, were used to design species\u2010specific microarray oligonucleotide probes (oligoprobes). The microarray also contained several oligoprobes selected from the <jats:italic>ORF31<\/jats:italic>, <jats:italic>US4<\/jats:italic>, and <jats:italic>US5 genes<\/jats:italic> of VZV, HSV\u20101, and HSV\u20102, respectively. The samples (from HSVs or OPVs) of ssDNAs for analyses were prepared by using asymmetric PCR followed by chemical labeling of ssDNA with Cy3 dye. DNA from 52 samples of various OPV species, two isolates of VZV, two of HSV\u20101, and three of HSV\u20102 were tested using the developed microarray assay; all tested viruses were accurately identified. To ensure the robustness of the microarray assay, three additional unrelated variola virus strains with unknown sequences of the <jats:italic>C23L\/B29R<\/jats:italic> and the <jats:italic>B19R<\/jats:italic> genes were tested. In each instance the microarray unambiguously identified them as <jats:italic>Variola virus<\/jats:italic> species. The results obtained in this study demonstrated that this new microarray method is a valuable tool for the rapid and accurate detection and differentiation of these important viral pathogens. J. Med. 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