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Poliovirus, adenovirus, and herpes simplex virus (HSV) induced cytopathology in Hep G2 cells. Measles virus caused no change in hepatocytes. Poliovirus infection did not affect cellular protein synthesis, and the peak of hepatocellular enzyme release coincided with the peak of virus release. The increase in adenovirus protein synthesis correlated with the decrease of transferrin synthesis, and enzyme release was not prominent. HSV induced viral protein synthesis with enhanced processing and inhibition of synthesis of \u03b11\u2010antitrypsin. The peak of enzyme release was later than the peak of virus release. In primary hepatocytes, poliovirus, adenovirus, and induced extensive cytopathology and enzyme release, and VZV caused cytopathology and significant but minute enzyme release. The ratio of lactate dehydrogenase to aspartate aminotransferase release was larger in poliovirus infection in both hepatocytes than in HSV or VZV infection. Although poliovirus and adenovirus are released by cytolysis and HSV and VZV are secreted by exocytosis of cytoplasmic vacuoles, enzyme release was independent of the type of virus release. Adenovirus showed strong cytotoxicity but did not modify the membrane nor cause enzyme release. Enzyme release was associated with modification of the surface membrane due to apoptosis with poliovirus and necrosis with HSV. Consequently hepatocellular injury by viral infection did not reflect the amount or pattern of hepatocellular enzyme release. J. Med. Virol. 79:413\u2013425, 2007. \u00a9 2007 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jmv.20783","type":"journal-article","created":{"date-parts":[[2007,2,20]],"date-time":"2007-02-20T21:48:14Z","timestamp":1172008094000},"page":"413-425","source":"Crossref","is-referenced-by-count":4,"title":["Infection and direct injury in human hepatocyte explants and a hepatoblastoma cell line due to hepatiticomimetic (non\u2010hepatitis) viruses"],"prefix":"10.1002","volume":"79","author":[{"given":"Jurairatana","family":"Phromjai","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nobuyasu","family":"Aiba","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mikiko","family":"Suzuki","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hitoshi","family":"Sato","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Terumi","family":"Takahara","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sachiko","family":"Kondo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kimiyasu","family":"Shiraki","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2007,2,20]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1097\/00006454-200108000-00008"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.40.3.1060-1062.2002"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1442-200X.1993.tb03068.x"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.50.1.202-212.1984"},{"key":"e_1_2_1_6_1","first-page":"68","article-title":"Varicella zoster virus infections in immunocompromised host: A review of the natural history and management","volume":"85","author":"Balfour HH","year":"1988","journal-title":"Am J Med"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/3-540-27320-4_2"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-3476(05)80593-1"},{"key":"e_1_2_1_9_1","first-page":"1721","volume-title":"Harrison's Principles of Internal Medicine","author":"Dienstag JL","year":"2001"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.36.11.3323-3326.1998"},{"key":"e_1_2_1_11_1","first-page":"740","article-title":"Coxsackie A 10 virus infection among infectious hepatitis contacts","volume":"93","author":"Embil JA","year":"1965","journal-title":"Can Med Ass J"},{"key":"e_1_2_1_12_1","doi-asserted-by":"crossref","first-page":"285","DOI":"10.1542\/peds.67.2.285","article-title":"Varicella hepatitis without neurologic symptoms or findings","volume":"67","author":"Ey JL","year":"1981","journal-title":"Pediatrics"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.3109\/00365549709008661"},{"key":"e_1_2_1_14_1","doi-asserted-by":"crossref","first-page":"6905","DOI":"10.1128\/jvi.71.9.6905-6912.1997","article-title":"Poliovirus Sabin type 1 neutralization epitopes recognized by immunoglobulin A monoclonal antibodies","volume":"71","author":"Fiore L","year":"1997","journal-title":"J Virol"},{"key":"e_1_2_1_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/hep.1840360616"},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.1001\/archinte.1983.00350040064008"},{"key":"e_1_2_1_17_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.95.19.11089"},{"key":"e_1_2_1_18_1","first-page":"575","article-title":"Coxsackie hepatitis in an adult, with ultrastructural demonstration of the virus","volume":"42","author":"Gregor GR","year":"1975","journal-title":"Mount Sinai J Med"},{"key":"e_1_2_1_19_1","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.56.1.250-257.1985"},{"key":"e_1_2_1_20_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.71.5.2403-2411.2005"},{"key":"e_1_2_1_21_1","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1128\/jvi.14.1.8-19.1974","article-title":"Regulation of herpesvirus macromolecular synthesis. 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