{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,20]],"date-time":"2026-09-20T06:10:55Z","timestamp":1789884655293,"version":"4.0.1"},"reference-count":53,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2007,1,23]],"date-time":"2007-01-23T00:00:00Z","timestamp":1169510400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2007,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>While the impact of the dengue viruses on liver function is prominent as shown by hepatomegaly, liver enzyme abnormality, occasional fulminant hepatic failure and histological changes including hepatocellular necrosis, significant debate exists as to the possible involvement of the predominant cell type in the liver, hepatocytes, in the disease process. To address this issue purified human primary hepatocytes were exposed to dengue virus serotype 2 and the production of de novo viral progeny was established by standard plaque assay, RT\u2010PCR and immunocytochemistry. To investigate the response of the primary hepatocytes to infection, the expression of a panel of 9 cytokine genes (IFN\u2010\u03b2, TRAIL, MCP\u20101, IL\u20106, IL\u20101\u03b2, IL\u20108, MIP\u20101\u03b1, MIP\u20101\u03b2, and RANTES) was semi\u2010quantitatively investigated by RT\u2010PCR and up\u2010regulation of TRAIL, MIP\u20101\u03b1, IFN\u2010\u03b2, MIP\u20101\u03b2, IL\u20108, and RANTES was observed in response to infection. The induction of IL\u20108 in response to infection was accompanied by the secretion of IL\u20108 as verified by ELISA assay. The ability of hepatocytes to be infected with dengue virus serotype 2 in vitro support evidence implicating human hepatocytes as a target cell in cases of dengue virus infection, and provide the first experimental evidence to support the large number of clinical studies that implicate the liver as a critical target organ in severe cases of dengue infection. J. Med. Virol. 79:300\u2013307, 2007. \u00a9 2007 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jmv.20798","type":"journal-article","created":{"date-parts":[[2007,1,23]],"date-time":"2007-01-23T20:12:32Z","timestamp":1169583152000},"page":"300-307","source":"Crossref","is-referenced-by-count":51,"title":["Infection of human primary hepatocytes with dengue virus serotype 2"],"prefix":"10.1002","volume":"79","author":[{"given":"Lukkana","family":"Suksanpaisan","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arturo","family":"Cabrera\u2010Hernandez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Duncan 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