{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,2]],"date-time":"2026-04-02T17:48:01Z","timestamp":1775152081208,"version":"3.50.1"},"reference-count":22,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2007,1,23]],"date-time":"2007-01-23T00:00:00Z","timestamp":1169510400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2007,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>A one\u2010step reverse transcription loop\u2010mediated isothermal amplification (RT\u2010LAMP) assay for the detection of norovirus (NV) was developed. In order to design primer sets for the detection of a wide range of NVs, NVs were categorized into three groups, that is, genogroup I (GI), prevalent GII, and minor GII; three sets of primers were developed for each group. Clinical specimens of patients suffering from enteric RNA viruses, such as NV, group A and C rotavirus, and sapovirus were examined using these primer sets. Various genotypes of NVs were detected in clinical specimens from patients infected with NV where no false positive reaction was observed with other enteric RNA viruses. Additionally, 88 samples of acute gastroenteritis outbreaks were analyzed by an RT\u2010LAMP assay and compared with the results of routine RT\u2010PCR. The results of the RT\u2010LAMP assay corresponded well to that of RT\u2010PCR. These findings suggest the practical application of the RT\u2010LAMP assay for the detection of NVs in clinical specimens. Consequently, the RT\u2010LAMP system and conventional detection kits (NVGI and NVGII detection kits; Eiken Chemical Co., Ltd., Japan) were compared. The detection rate of the prevalent and minor GII primer sets was similar to that of the conventional NVGII kit, while the detection rate of the GI primer set is different because it can detect several genotypes better than the conventional NVGI kit. This is an initial report that the RT\u2010LAMP system is able to detect NVs in clinical specimens within a wide range. J. Med. Virol. 79:326\u2013334, 2007. \u00a9 2007 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jmv.20802","type":"journal-article","created":{"date-parts":[[2007,1,23]],"date-time":"2007-01-23T20:11:05Z","timestamp":1169583065000},"page":"326-334","source":"Crossref","is-referenced-by-count":61,"title":["Evaluation and application of reverse transcription loop\u2010mediated isothermal amplification for detection of noroviruses"],"prefix":"10.1002","volume":"79","author":[{"given":"Tomoko","family":"Yoda","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yasuhiko","family":"Suzuki","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kenji","family":"Yamazaki","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Naomi","family":"Sakon","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masashi","family":"Kanki","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ikuko","family":"Aoyama","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Teizo","family":"Tsukamoto","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2007,1,23]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1086\/499315"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1096-9071(199605)49:1<70::AID-JMV12>3.0.CO;2-6"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1093\/aje\/154.7.666"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1086\/341085"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.44.4.1376-1381.2006"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/0168-1702(95)00041-N"},{"key":"e_1_2_1_8_1","unstructured":"Infectious Agents Surveillance Report (IASR).2005.Outbreaks of norovirus infection September 2003\u2013October 2005.26:323\u2032\u2013324\u2032[Online]http:\/\/idsc.nih.go.jp\/iasr\/"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.41.4.1548-1557.2003"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.42.7.2988-2995.2004"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1077905"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.20419"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0166-0934(01)00404-9"},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.2001.5921"},{"key":"e_1_2_1_15_1","doi-asserted-by":"publisher","DOI":"10.1006\/mcpr.2002.0415"},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/28.12.e63"},{"key":"e_1_2_1_17_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.77.4.2789-2798.2003"},{"key":"e_1_2_1_18_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.42.1.257-263.2004"},{"key":"e_1_2_1_19_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.43.6.2895-2903.2005"},{"key":"e_1_2_1_20_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.42.5.1956-1961.2004"},{"key":"e_1_2_1_21_1","doi-asserted-by":"publisher","DOI":"10.1007\/s007050050020"},{"key":"e_1_2_1_22_1","doi-asserted-by":"publisher","DOI":"10.1136\/bmj.318.7190.1046"},{"key":"e_1_2_1_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.virol.2005.11.015"}],"container-title":["Journal of Medical Virology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjmv.20802","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jmv.20802","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,15]],"date-time":"2023-11-15T17:06:10Z","timestamp":1700067970000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jmv.20802"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2007,1,23]]},"references-count":22,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2007,3]]}},"alternative-id":["10.1002\/jmv.20802"],"URL":"https:\/\/doi.org\/10.1002\/jmv.20802","archive":["Portico"],"relation":{},"ISSN":["0146-6615","1096-9071"],"issn-type":[{"value":"0146-6615","type":"print"},{"value":"1096-9071","type":"electronic"}],"subject":[],"published":{"date-parts":[[2007,1,23]]}}}