{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,3,27]],"date-time":"2025-03-27T15:54:47Z","timestamp":1743090887760},"reference-count":40,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2007,4,24]],"date-time":"2007-04-24T00:00:00Z","timestamp":1177372800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2007,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Human T\u2010cell lymphotropic virus type I (HTLV\u2010I) is the etiologic agent of HTLV\u2010I\u2010associated myelopathy\/tropical spastic paraparesis (HAM\/TSP). High HTLV\u2010I provirus load and <jats:italic>tax<\/jats:italic> mRNA level have been suggested as predictors of disease progression in patients with HAM\/TSP, but little is known about the temporal variation in patients. To clarify the role of high proviral and <jats:italic>tax<\/jats:italic> mRNA loads and their fluctuations in the pathogenesis of HAM\/TSP, we measured proviral load and <jats:italic>tax<\/jats:italic> mRNA in serially collected peripheral blood mononuclear cells (PBMCs) from nine patients with HAM\/TSP during a long\u2010term follow\u2010up, by use of real\u2010time polymerase chain reaction using tax primers. The real\u2010time PCR quantitation revealed a wide range of variation of proviral loads (7.82\u201397.13 copies per 100 PBMCs) and tax mRNA (0.20\u2013245.30 copies) among HAM\/TSP patients. Patients showed three different patterns of HTLV\u2010I tax mRNA loads during the course of the disease. Tax mRNA load showed a separate evolution with respect to the disease. The dynamic patterns of proviral load and mRNA Tax expression suggest that only the permanent presence of a basal level of tax mRNA, rather than the tax mRNA load, is related to the development of HAM\/TSP. To our knowledge, this is the first longitudinal study to determine <jats:italic>tax<\/jats:italic> mRNA expression at different clinical stages. J. Med. Virol. 79: 782\u2013790, 2007. \u00a9 2007 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jmv.20844","type":"journal-article","created":{"date-parts":[[2007,4,24]],"date-time":"2007-04-24T22:47:52Z","timestamp":1177454872000},"page":"782-790","source":"Crossref","is-referenced-by-count":14,"title":["Temporal dynamics of human T\u2010lymphotropic virus type I <i>tax<\/i> mRNA and proviral DNA load in peripheral blood mononuclear cells of human T\u2010lymphotropic virus type I\u2010associated myelopathy 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