{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,7,5]],"date-time":"2024-07-05T05:58:38Z","timestamp":1720159118134},"reference-count":34,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2007,3,26]],"date-time":"2007-03-26T00:00:00Z","timestamp":1174867200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2007,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Fourteen serum samples obtained from hepatitis B virus (HBV) chronic carriers and patients recovered from hepatitis B infection were used with four sodium dodecyl sulfate\u2010treated enzyme\u2010linked immunosorbent assay (ELISA) plates available commercially, and one self\u2010prepared HBcAg analog for evaluation of anti\u2010HBe subclass pattern absorbance. The self\u2010prepared plates had the best performance and were thus used for samples obtained from 104 (60 male and 44 female) HBV chronic carriers and 439 (247 male and 192 female) recovered individuals. Tests for aspartate aminotransferase (AST) and alanine aminotransferase (ALT) were also carried out in 21 of the subjects (&gt;25 IU\/ml). Statistical comparison of these patients with elevated ALT\/AST levels with other ALT\/AST\u2010normal chronic carriers revealed no significant differences in the anti\u2010HBe OD, although the mean optical density (OD) of patients with elevated ALT\/AST levels was higher. The results suggest that the anti\u2010HBe IgG subclass profiles in the chronic carriers did not change with inflammation of the liver, and were independent of sex and age. In contrast to previous anti\u2010HBc findings, the distribution pattern of anti\u2010HBe subclasses in HBV chronic carriers was IgG1\u2009&gt; IgG4\u2009&gt;\u2009IgG3 while in the recovered individuals it was IgG1\u2009&gt;\u2009IgG3\u2009&gt;\u2009IgG4, for both males and females. Subclasses IgG1 and IgG2 were the most and least prevalent isotypes, respectively, in both study groups. The results of the study suggest that induction of IgG1 and\/or IgG3 antibodies is important for effective virus neutralization, while IgG2 antibodies are of limited importance. Significantly higher OD values for anti\u2010HBe IgG4 were observed when comparing samples from the chronic carriers and recovered individuals, which may reflect the effects of persistence. Further, in contrast to previous anti\u2010HBs results, the concentrations of total IgG and IgG1 were higher in the samples from chronic carriers relative to those from recovered individuals. J. Med. Virol. 79:495\u2013502, 2007. \u00a9 2007 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jmv.20852","type":"journal-article","created":{"date-parts":[[2007,3,26]],"date-time":"2007-03-26T20:14:50Z","timestamp":1174940090000},"page":"495-502","source":"Crossref","is-referenced-by-count":5,"title":["Utilizing self\u2010prepared ELISA plates for a cross\u2010population study of different anti\u2010HBe IgG subclass profiles"],"prefix":"10.1002","volume":"79","author":[{"given":"Lina","family":"Wang","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tzu\u2010Hsin","family":"Tsai","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chien\u2010Fu","family":"Huang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mei\u2010Shang","family":"Ho","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ding\u2010Bang","family":"Lin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yung\u2010Chyuan","family":"Ho","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shih\u2010Shen","family":"Lin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"James Cheng\u2010Chung","family":"Wei","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ming\u2010Chih","family":"Chou","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chi\u2010Chiang","family":"Yang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2007,3,26]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"crossref","first-page":"722","DOI":"10.4049\/jimmunol.130.2.722","article-title":"Serologic aspects of IgG4 antibodies. 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