{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,5]],"date-time":"2026-08-05T00:07:13Z","timestamp":1785888433315,"version":"3.56.0"},"reference-count":33,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2007,3,26]],"date-time":"2007-03-26T00:00:00Z","timestamp":1174867200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2007,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>A new method was developed for detection of human papillomavirus (HPV) by loop\u2010mediated isothermal amplification (LAMP), which was compared with the polymerase chain reaction (PCR), and real\u2010time PCR for specificity and sensitivity. All initial validation studies with the control DNA proved to be type\u2010specific. In order to evaluate the reliability of HPV type\u2010specific LAMP detecting HPV DNA from clinical samples, tissue specimens were obtained from 27 patients with external genital polypoid lesions. The histologic diagnoses included condyloma acuminatum (n\u2009=\u200921), bowenoid papulosis (n\u2009=\u20092), seborrheic keratosis (n\u2009=\u20092), epidermolytic acanthoma (n\u2009=\u20091), and hairy nymphae (n\u2009=\u20091). HPV\u20106 DNA and HPV\u201011 DNA were detected in 18 and 3 of 21 condylomata acuminata, respectively, and there was no simultaneous infection. HPV\u201016 DNA was detected in one of two bowenoid papuloses. HPV DNA was not detected in the seborrheic keratoses, epidermolytic acanthoma, and hairy nymphae. These results correlated perfectly with those from real\u2010time PCR analysis. Most positive samples contained high copy numbers of HPV DNA. HPV\u201011 DNA was detected in one case that could not be detected by PCR. The average reaction time was about 59 min. There was a linear correlation between the genome quantity and reaction time to reach the threshold. The LAMP method has an additional advantage as a quantitative method, and is superior in terms of sensitivity, specificity, rapidity, and simplicity, and can potentially be a valuable tool for the detection of HPV DNA. J. Med. Virol. 79:605\u2013615, 2007. \u00a9 2007 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jmv.20858","type":"journal-article","created":{"date-parts":[[2007,3,26]],"date-time":"2007-03-26T20:17:26Z","timestamp":1174940246000},"page":"605-615","source":"Crossref","is-referenced-by-count":63,"title":["Loop\u2010mediated isothermal amplification method for detection of human papillomavirus type 6, 11, 16, and 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