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Two hundred and thirty\u2010seven liquid based cytology samples were collected from women attending colposcopy clinics, DNA was extracted, and presence of virus determined by PCR\u2010enzyme immunoassay. Quantitative real\u2010time PCR was used to determine viral load for 70 HPV\u201016 positive single infections. Viral load was expressed as the ratio of copies of the viral L1 gene to copies of the human beta\u2010globin gene. Measurements varied from 0.019 to 4,194 HPV genomes per cell. Our data demonstrate that in cervical neoplasia, HPV load tends to correlate with disease severity, but that the number of viral genomes\/cell varies considerably within histological grades. This variation within disease grades currently limits the clinical utility of viral load measurement.<\/jats:p>","DOI":"10.1002\/jmv.20875","type":"journal-article","created":{"date-parts":[[2007,7,3]],"date-time":"2007-07-03T01:39:51Z","timestamp":1183426791000},"page":"1366-1369","source":"Crossref","is-referenced-by-count":20,"title":["Variation in human papillomavirus type\u201016 viral load within different histological grades of cervical neoplasia"],"prefix":"10.1002","volume":"79","author":[{"given":"A.N.","family":"Fiander","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K.W.","family":"Hart","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.J.","family":"Hibbitts","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G.C.","family":"Rieck","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.J.","family":"Tristram","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R.W.","family":"Beukenholdt","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"N.G.","family":"Powell","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2007,7,2]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjc.6602648"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.44.5.1755-1762.2006"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.90010"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1067\/mob.2003.439"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.20062"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.39.9.3204-3212.2001"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjc.6603245"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.35.3.791-795.1997"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(00)02401-6"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcv.2005.05.006"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.20480"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjc.6602436"},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.40.3.886-891.2002"},{"key":"e_1_2_1_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0215(19961211)68:6<766::AID-IJC13>3.0.CO;2-Z"},{"key":"e_1_2_1_16_1","first-page":"55","article-title":"Epidemiology of cervical human papillomavirus infections","volume":"186","author":"Schiffman MH","year":"1994","journal-title":"Curr Top Microbiol Immunol"},{"key":"e_1_2_1_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.10846"},{"key":"e_1_2_1_18_1","first-page":"1038","article-title":"Determinants of human papillomavirus load among women with histological cervical intraepithelial neoplasia 3: Dominant impact of surrounding low\u2010grade lesions","volume":"12","author":"Sherman ME","year":"2003","journal-title":"Cancer Epidemiol Biomarkers Prev"},{"key":"e_1_2_1_19_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.37.4.1030-1034.1999"},{"key":"e_1_2_1_20_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.10232"},{"key":"e_1_2_1_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(00)02402-8"},{"key":"e_1_2_1_22_1","doi-asserted-by":"publisher","DOI":"10.1136\/jcp.54.5.377"}],"container-title":["Journal of Medical Virology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjmv.20875","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jmv.20875","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,14]],"date-time":"2023-11-14T19:38:06Z","timestamp":1699990686000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jmv.20875"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2007,7,2]]},"references-count":21,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2007,9]]}},"alternative-id":["10.1002\/jmv.20875"],"URL":"https:\/\/doi.org\/10.1002\/jmv.20875","archive":["Portico"],"relation":{},"ISSN":["0146-6615","1096-9071"],"issn-type":[{"value":"0146-6615","type":"print"},{"value":"1096-9071","type":"electronic"}],"subject":[],"published":{"date-parts":[[2007,7,2]]}}}