{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,7]],"date-time":"2025-11-07T13:04:31Z","timestamp":1762520671695},"reference-count":41,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2001,10,23]],"date-time":"2001-10-23T00:00:00Z","timestamp":1003795200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2001,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Human papillomavirus (HPV) infection is associated with cervical cancer. The E2 and E1 papillomavirus proteins are expressed at the early stage of infection and regulate DNA replication. The E2 protein activates and represses transcription from different HPVs promoters. At some stage when viral DNA gets integrated into the cellular genome, the E2 gene is disrupted or inactivated. This event leads to a derepression of the E6 and E7 viral oncogenes. These viral proteins are required normally for the maintenance of the malignant phenotype. Therefore, the E2, E6, and E7 proteins are present in all patients infected by papillomavirus. In this study, the association of antibody levels against E2, E6, and E7 proteins of HPV types 16, 18, and 6 was determined in relation to the presence of HPV DNA at the initial stages of HPV infection. Serum samples from 172 women with HPV infection, determined by Papanicolau (Pap) smears and colposcopy, were tested. Elevated antibody titers against E2 protein from the HPV 6 and HPV 16 were detected in 46.42 and 66.96% of the patients, respectively. Antibodies against the E7 and E6 proteins of HPV 16 were found in 51.78 and 36.60% of the patients, respectively. Antibodies against the E6 and E7 proteins of HPV 18 were 35 and 45%, respectively. A statistical difference was found for antibody titers against the E2, E6, and E7 proteins between patients with papillomavirus DNA and controls cases who had no cytological abnormalities and no HPV DNA. Sera titers were 1\/500 for patients HPV positive and 1\/50 for control individuals. Antibodies titers against E6 and E7 proteins were also examined in patients at 6 and 24 months after cryosurgery. In these patients, a slight decrease in the antibody level against the E2, E6, and E7 proteins was found. No correlation was found between age and number of sexual partners, with serum positivity to the E2, E6, and E7 papillomavirus proteins. These data suggest that antibodies against the E2, E6, and E7 proteins are good candidates for use as markers for monitoring cervical HPV infections. J. Med. Virol. 65:736\u2013744, 2001. \u00a9 2001 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jmv.2098","type":"journal-article","created":{"date-parts":[[2002,8,25]],"date-time":"2002-08-25T21:17:12Z","timestamp":1030310232000},"page":"736-744","source":"Crossref","is-referenced-by-count":15,"title":["Antibodies against human papillomavirus (HPV) type 16 and 18 E2, E6 and E7 proteins in sera: Correlation with presence of papillomavirus DNA"],"prefix":"10.1002","volume":"65","author":[{"given":"Ricardo","family":"Rosales","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mario","family":"L\u00f3pez\u2010Contreras","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Roberto Risco","family":"Cortes","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2001,10,23]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s100960050240"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1086\/315498"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1006\/gyno.1995.1130"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.86.10.3838"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.2910430109"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.80.12.3812"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1006\/gyno.1996.0099"},{"key":"e_1_2_1_9_1","first-page":"59","article-title":"Characterization of the humoral immune response to genital papillomaviruses","volume":"7","author":"Galloway D","year":"1990","journal-title":"Mol Biol Med"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1099\/0022-1317-73-6-1395"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1346-8138.2000.tb02126.x"},{"key":"e_1_2_1_12_1","doi-asserted-by":"crossref","first-page":"2215","DOI":"10.1128\/jvi.62.6.2115-2123.1988","article-title":"Identification of immunoreactive antigens of human papillomavirus type 6b by using Escherichia coli\u2010expressed fuson proteins","volume":"62","author":"Jenison S","year":"1988","journal-title":"J Virol"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1093\/jnci\/81.22.1698"},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1525-1438.1999.09891.x"},{"key":"e_1_2_1_15_1","first-page":"101","article-title":"High\u2010risk human papillomavirus is sexually transmitted: evidence from a follow\u2010up study of virgins starting sexual activity (intercourse)","volume":"10","author":"Kjaer S","year":"2001","journal-title":"Cancer Epidemiol Biomark Prev"},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.2910480509"},{"key":"e_1_2_1_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.1890370306"},{"key":"e_1_2_1_18_1","doi-asserted-by":"crossref","first-page":"2684","DOI":"10.1128\/jvi.61.9.2684-2690.1987","article-title":"Identification of human papillomavirus type 6b L1 open reading frame protein in condylomas and corresponding antibodies in human sera","volume":"61","author":"Li C","year":"1987","journal-title":"J Virol"},{"key":"e_1_2_1_19_1","doi-asserted-by":"crossref","first-page":"606","DOI":"10.1128\/jvi.62.2.606-609.1988","article-title":"Identification of the human papilomavirus E2 protein in genital tract tissue","volume":"62","author":"Li CCH","year":"1988","journal-title":"J Virol"},{"key":"e_1_2_1_20_1","volume-title":"Introduction to the theory of statistics","author":"Mood AM","year":"1974"},{"key":"e_1_2_1_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/0042-6822(92)90453-V"},{"key":"e_1_2_1_22_1","first-page":"362","article-title":"The causal link between HPV and cervical cancer and its implications for prevention of cervical cancer","volume":"30","author":"Mu\u00f1oz N","year":"1996","journal-title":"Bull Pan Am Health Org"},{"key":"e_1_2_1_23_1","doi-asserted-by":"publisher","DOI":"10.1590\/S0036-36341997000400005"},{"key":"e_1_2_1_24_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.2910520513"},{"key":"e_1_2_1_25_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF01309480"},{"key":"e_1_2_1_26_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.2910540417"},{"key":"e_1_2_1_27_1","doi-asserted-by":"publisher","DOI":"10.1007\/s007050050050"},{"key":"e_1_2_1_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/0140-6736(90)90793-5"},{"key":"e_1_2_1_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/0959-8049(96)00005-6"},{"key":"e_1_2_1_30_1","first-page":"341","article-title":"Human papillomavirus\u2010related serological markers of invasive cervical carcinoma in Brazil","volume":"3","author":"Sun Y","year":"1994","journal-title":"Cancer Epidemiol Biomark Prev"},{"key":"e_1_2_1_31_1","first-page":"935","article-title":"Serum antibodies to human papillomavirus 16 proteins in women from Brazil with invasive cervical carcinoma","volume":"8","author":"Sun Y","year":"1999","journal-title":"Cancer Epidemiol Biomark Prev"},{"key":"e_1_2_1_32_1","first-page":"90","article-title":"Functional analysis of E2\u2010mediated repression of the HPV18 P105 promoter","volume":"3","author":"Thierry F","year":"1991","journal-title":"New Biol"},{"key":"e_1_2_1_33_1","first-page":"611","article-title":"Characterization of human papillomavirus types in condylomata acuminata in children by in situ hybridization","volume":"56","author":"Vallejos H","year":"1987","journal-title":"Lab Invest"},{"key":"e_1_2_1_34_1","doi-asserted-by":"publisher","DOI":"10.1136\/sti.74.5.354"},{"key":"e_1_2_1_35_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.2910550515"},{"key":"e_1_2_1_36_1","doi-asserted-by":"crossref","first-page":"122","DOI":"10.1128\/cdli.4.2.122-126.1997","article-title":"Prevalence of antibodies to human papillomavirus (HPV) type 16 virus\u2010like particles in relation to cervical HPV infection among college women","volume":"4","author":"Viscidi R","year":"1997","journal-title":"Clin Diagn Lab Immunol"},{"key":"e_1_2_1_37_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1096-9896(199909)189:1<12::AID-PATH431>3.0.CO;2-F"},{"key":"e_1_2_1_38_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0215(20000201)85:3<353::AID-IJC10>3.0.CO;2-M"},{"key":"e_1_2_1_39_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0215(20000315)85:6<815::AID-IJC14>3.0.CO;2-X"},{"key":"e_1_2_1_40_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0215(20000201)85:3<313::AID-IJC3>3.0.CO;2-W"},{"key":"e_1_2_1_41_1","doi-asserted-by":"publisher","DOI":"10.1093\/jnci\/81.22.1680"},{"key":"e_1_2_1_42_1","first-page":"245","volume-title":"The papillomaviruses","author":"zur Hausen H","year":"1987"}],"container-title":["Journal of Medical Virology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjmv.2098","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jmv.2098","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,19]],"date-time":"2023-11-19T15:13:02Z","timestamp":1700406782000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jmv.2098"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2001,10,23]]},"references-count":41,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2001,12]]}},"alternative-id":["10.1002\/jmv.2098"],"URL":"https:\/\/doi.org\/10.1002\/jmv.2098","archive":["Portico"],"relation":{},"ISSN":["0146-6615","1096-9071"],"issn-type":[{"value":"0146-6615","type":"print"},{"value":"1096-9071","type":"electronic"}],"subject":[],"published":{"date-parts":[[2001,10,23]]}}}