{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,13]],"date-time":"2025-09-13T16:14:48Z","timestamp":1757780088165},"reference-count":58,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2007,9,13]],"date-time":"2007-09-13T00:00:00Z","timestamp":1189641600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2007,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The Epstein\u2013Barr virus (EBV) early antigen (EA) complex consists of multiple proteins with relevance for diagnosis of acute, chronic and malignant EBV related diseases, including nasopharyngeal carcinoma (NPC). In a recent study, it was found that the molecular diversity of EBV\u2010specific IgG and IgA antibody responses in NPC patients and demonstrated that these reflect independent B\u2010cell triggering leading to distinct EBV antigen\u2010recognition profiles. The fine\u2010specificity of NPC\u2010related IgG and IgA responses was explored further against defined recombinant and synthetic EBV\u2010EA antigens using immunofluorescence, immunoblot and ELISA techniques and determined their diagnostic value in a large panel of sera from NPC (n\u2009=\u2009154), non\u2010NPC tumor patients (n\u2009=\u2009133), acute mononucleosis patients (n\u2009=\u200970) and healthy EBV carriers (n\u2009=\u2009259). Individual recombinant EBV\u2010EA markers yielded sensitivity\/specificity values not exceeding 86%, whereas selected EA\u2010specific peptide epitopes were rather poorly recognized by IgG and IgA antibodies in NPC sera. Surprisingly, we found that a \u201clow salt\u201d native EA\u2010protein extract reproducibly prepared from purified nuclei of EA\u2010induced HH514 cells, and containing characteristic EA(D)\u2010polypeptides, such as p47\u201054 (BMRF1), p138 (BALF2), p55\u2010DNAse (BGLF5), and p65\u2010TK (BXLF1), but without viral capsid (VCA) or nuclear antigen (EBNA) reactivity, gave highest sensitivity (90.4%) and specificity (95.5%) values for NPC diagnosis in both IgG and IgA ELISA. The data support further the notion that EBV\u2010EA reactive IgG and IgA antibodies in NPC patients are directed against distinct conformational and\u2014in part\u2014linear epitopes on EBV\u2010specific proteins, barely recognized in other EBV\u2010related syndromes. The use of a defined native EBV EA\u2010specific antigen opens the way to further improve serological diagnosis of NPC. J. Med. Virol. 79:1710\u20131721, 2007. \u00a9 2007 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jmv.20987","type":"journal-article","created":{"date-parts":[[2007,9,13]],"date-time":"2007-09-13T16:48:01Z","timestamp":1189702081000},"page":"1710-1721","source":"Crossref","is-referenced-by-count":30,"title":["Native early antigen of Epstein\u2013Barr virus, a promising antigen for diagnosis of nasopharyngeal carcinoma"],"prefix":"10.1002","volume":"79","author":[{"given":"Dewi K.","family":"Paramita","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jajah","family":"Fachiroh","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wayan T.","family":"Artama","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eric","family":"van Benthem","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sofia 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