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During the present study, an early passage RS\u201012 containing its virulent pathogenic phenotype, was characterized genetically. Its F, SH and HN genes were isolated by RT\u2010PCR amplification and sequenced. It is quite evident that RS\u201012 belongs to genotype H, closely related to European strains but distinguishable from Asian strains. The deduced amino acid sequences of HN and F proteins that comprise immunogenic epitopes, were compared to other vaccine and wild strains. The multiple sequence alignment revealed that the RS\u201012 has isoleucine and aspartic acid at positions 269 and 523 of its F and HN proteins, respectively, which could differentiate RS\u201012 from other available sequences. This isolate has trivial variations in the major antigenic sites of HN protein. The frequency and pattern of F and HN glycosylation sites seems to be similar to most other strains. It seems that the mumps regional outbreak during 1986 in Iran was caused by genotype H and this strain has been spreading in countries surrounding the Caspian sea for over 17 years. These data support the previous results that RS\u201012 could be an efficient vaccine, especially in the Middle East. This is the first genotype report from Iranian isolates and provides strong data on the molecular epidemiology of mumps in Iran, the Middle East, Central Asia, Russia and other countries of this region. J. Med. Virol. 80:702\u2013710, 2008. \u00a9 2008 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jmv.21087","type":"journal-article","created":{"date-parts":[[2008,2,23]],"date-time":"2008-02-23T02:46:38Z","timestamp":1203734798000},"page":"702-710","source":"Crossref","is-referenced-by-count":6,"title":["Genetic characterization of RS\u201012 (S\u201012), an Iranian isolate of mumps virus, by sequence analysis and comparative genomics of F, SH, and HN 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