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Each inoculum contained 5\u2009\u00d7\u200910<jats:sup>4<\/jats:sup> genome equivalents of HEV. After infection, serum and fecal samples were collected serially and the levels of alanine aminotransferase (ALT) and anti\u2010HEV IgG and IgM in sera, and HEV RNA in fecal samples, were measured. Liver biopsies were carried out. All the infected monkeys (12\/12) developed anti\u2010HEV IgG and exhibited fecal shedding of virus. IgM was detected in 11 of 12, and ALT elevation occurred about 2\u20136 weeks post\u2010inoculation in 10 of 12, infected monkeys. Hepatic histopathology was consistent with acute viral hepatitis and the ORF2 antigen of HEV was detected in the granular cytoplasm of hepatocytes by immunohistochemistry. After recovery from their initial HEV infection, the monkeys were challenged with a heterologous genotype or heterologous source of HEV and monitored for hepatitis and fecal shedding. Previous infection with HEV completely or partially protected against subsequent challenge with a heterologous virus, because 7 of 11 monkeys did not develop HEV infection or shed virus in the feces, and none of them developed hepatitis or exhibited ALT elevation or liver biopsy findings of hepatitis. In conclusion, previous HEV infection may give rise to cross\u2010genotype and cross\u2010host\u2010species protection. J. Med. Virol. 80:824\u2013832, 2008. \u00a9 2008 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jmv.21140","type":"journal-article","created":{"date-parts":[[2008,3,21]],"date-time":"2008-03-21T23:17:31Z","timestamp":1206141451000},"page":"824-832","source":"Crossref","is-referenced-by-count":54,"title":["Cross\u2010protection of hepatitis E virus genotypes 1 and 4 in rhesus macaques"],"prefix":"10.1002","volume":"80","author":[{"given":"Weijin","family":"Huang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huayuan","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tim 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