{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,10]],"date-time":"2026-02-10T14:18:18Z","timestamp":1770733098190,"version":"3.49.0"},"reference-count":34,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2008,3,21]],"date-time":"2008-03-21T00:00:00Z","timestamp":1206057600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2008,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The aim of the study was to evaluate the mRNA expression of four relevant ABC\u2010transporter genes [MDR1 (P\u2010glycoprotein; Pgp), MRP1, MRP4, and MRP5] in HIV\u2010positive individuals failing treatment and analyze the association between the levels of their expression and viral load, CD4 cell count, and therapeutic history. Ninety\u2010eight HIV\u2010positive samples and 20 samples from healthy donors were analyzed, retrospectively. Peripheral blood mononuclear cells (PBMCs) from HIV1\u2010positive individuals were collected at the time of virological failure. Expression of mRNA of Pgp, MRP1, MRP4, and MRP5 in PBMCs was evaluated by real\u2010time PCR. A high inter\u2010individual variability was observed in both HIV\u2010positive individuals and healthy donors but the expression levels of all mRNA analyzed were significantly higher in the HIV\u2010infected group (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.05). A weak but significant inverse correlation was observed between CD4 cell counts and expression levels of MRP4 and MRP5. Comparison of mRNA expression between individuals with different therapeutic histories showed that expression of MRP4 and MRP5 genes in patients who were both protease inhibitor (PI) and non\u2010nucleoside reverse transcriptase inhibitor (NNRTI)\u2010experienced was significantly higher than in patients who were PI experienced but NNRTI\u2010na\u00efve. In conclusion, the mRNA expression of Pgp, MRP1, MRP4, and MRP5 varies among HIV\u2010infected patients and healthy donors but is significantly higher in HIV\u2010positive patients than in donors. The expression of MRP4 and MRP5 seems to correlate with CD4 cell counts. The same protein seems to be overexpressed in patients receiving NNRTIs. J. Med. Virol. 80:766\u2013771, 2008. \u00a9 2008 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jmv.21152","type":"journal-article","created":{"date-parts":[[2008,3,21]],"date-time":"2008-03-21T23:16:45Z","timestamp":1206141405000},"page":"766-771","source":"Crossref","is-referenced-by-count":36,"title":["Expression levels of MDR1, MRP1, MRP4, and MRP5 in peripheral blood mononuclear cells from HIV infected patients failing antiretroviral 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