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Infection with DENV can lead to a broad clinical spectrum, ranging from sub\u2010clinical infection or an influenza\u2010like disease known as dengue fever (DF) to a severe, sometimes fatal, disease characterized by hemorrhage and plasma leakage that can lead to shock, known as dengue hemorrhagic fever\/dengue shock syndrome (DHF\/DSS). The diagnosis of dengue is routinely accomplished by serologic assays, such as IgM and IgG ELISAs, as well as HI tests, analyzing serum samples obtained from patients with at least 7 days of symptoms onset. These tests cannot be used for diagnosis during the early symptomatic phase. In addition, antibodies against dengue are broad reactive with other flaviviruses. Therefore, a specific diagnostic method for acute DENV infection is of great interest. In that sense, the real\u2010time RT\u2010PCR has become an important tool that can be used for early and specific detection of dengue virus genome in human serum samples. This study describes a simple, specific, and sensitive real\u2010time RT\u2010PCR for early diagnosis of dengue virus infection. J. Med. Virol. 80:1426\u20131433, 2008. \u00a9 2008 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jmv.21203","type":"journal-article","created":{"date-parts":[[2008,6,12]],"date-time":"2008-06-12T21:47:12Z","timestamp":1213307232000},"page":"1426-1433","source":"Crossref","is-referenced-by-count":33,"title":["A simple one\u2010step real\u2010time RT\u2010PCR for diagnosis of dengue virus infection"],"prefix":"10.1002","volume":"80","author":[{"given":"Harryson Wings Godoy","family":"dos Santos","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Telma Regina Ramos Silva","family":"Poloni","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kelly Paula","family":"Souza","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vanessa Danielle 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