{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,8,7]],"date-time":"2024-08-07T21:52:16Z","timestamp":1723067536409},"reference-count":34,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2008,8,19]],"date-time":"2008-08-19T00:00:00Z","timestamp":1219104000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2008,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>To evaluate the neutralizing antibody activity of a human sera panel against seven strains of the homotypic virus. Sera were collected from DENV\u20103 immune individuals. Two DENV\u20103 genotypes and strains isolated at different time\u2010points during the 2000 and 2001\u20132002 Havana epidemics were included. A panel of 20 late convalescent sera collected 16\u201318 months after acute illness from DF and DHF patients are studied. These individuals were infected during the 2001\u20132002 Havana DENV\u20103 epidemic. All but four sera collected from DF cases had a secondary DENV\u20101\/DENV\u20103 infection. Sera neutralizing antibody titer against the seven DENV\u20103 strains were determined by plaque reduction neutralization technique. Sera samples were tested simultaneously. Studied sera showed higher levels of neutralizing antibodies to DENV\u20103 strains of genotype III compared to genotype V. Interesting, higher levels of neutralizing antibodies were detected to DENV\u20103 strain isolated at the end of the epidemic 2001\u20132002. An increased tendency of GMT of neutralizing antibodies according to epidemic evolution was observed for the 2001\u20132002 outbreak. In general, antibody levels in sera collected from DF cases were higher. Differences in the neutralization capacity of immune DENV\u20103 sera tested against two homologous genotypes including strains of the same genotype are demonstrated. Observed results suggest that virus changed in the course of the epidemic. The implications of this finding in terms of dengue pathogenesis and vaccine development need to be considered. J. Med. Virol. 80:1783\u20131789, 2008. \u00a9 2008 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jmv.21234","type":"journal-article","created":{"date-parts":[[2008,8,21]],"date-time":"2008-08-21T15:32:09Z","timestamp":1219332729000},"page":"1783-1789","source":"Crossref","is-referenced-by-count":10,"title":["Neutralizing antibody response variation against dengue 3 strains"],"prefix":"10.1002","volume":"80","author":[{"given":"Mayling","family":"Alvarez","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alequis","family":"Pavon\u2010Oro","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rosmari","family":"Rodriguez\u2010Roche","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L\u00eddice","family":"Bernardo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lu\u00eds","family":"Morier","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lizet","family":"Sanchez","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Angel M.","family":"Alvarez","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria G.","family":"Guzm\u00e1n","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2008,8,21]]},"reference":[{"key":"e_1_2_1_2_1","first-page":"49","article-title":"Improved dengue virus plaque formation on BHK21 and LLCMK2 Cells: Evaluation of some factors","volume":"29","author":"Alvarez M","year":"2005","journal-title":"Dengue Bull"},{"key":"e_1_2_1_3_1","doi-asserted-by":"crossref","unstructured":"AlvarezM Rodriguez\u2010RocheR BernardoL VazquezS MorierL GonzalezD CastroO KouriG HalsteadSB GuzmanMG.2006.Dengue hemorrhagic Fever caused by sequential dengue1\u20133virus infections over a long time interval: Havana epidemic 2001\u20132002.Am J Trop Med Hyg75:1113\u20131117.","DOI":"10.4269\/ajtmh.2006.75.1113"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.77.7.3929-3938.2003"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1590\/S0074-02761990000300012"},{"key":"e_1_2_1_6_1","first-page":"83","volume-title":"Factors in the emergence of arbovirus diseases","author":"Gubler DJ","year":"1997"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(01)00171-2"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1201-9712(99)90033-4"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(00)02303-5"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.239.4839.476"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0065-3527(03)60011-4"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.146.1.201"},{"key":"e_1_2_1_13_1","first-page":"311","article-title":"Observations related to pathogenesis of dengue hemorrhagic fever. 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