{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,11]],"date-time":"2025-12-11T20:34:26Z","timestamp":1765485266125},"reference-count":30,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2008,10,21]],"date-time":"2008-10-21T00:00:00Z","timestamp":1224547200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2008,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Jun\u00edn arenavirus is the etiologic agent of Argentine hemorrhagic fever. Due to its morbidity and high mortality rate in untreated cases, this endemic disease is of mandatory report in Argentina. Secure and accurate diagnostic methods are needed for the epidemiological surveillance of the disease. Current assays rely on antigens prepared from lysates of virus infected mammalian cells. The bio\u2010safety issue related to the manipulation of large quantities of virus restricts such antigen production to laboratories with the appropriate containment facilities. In this report, we describe the development of an enzyme linked immunosorbent assay for the etiologic confirmation of the disease, based on recombinant antigens expressed in insect cells. Eight different variables of the assay were optimized with the Taguchi approach for experimental design (L18 design, seven three\u2010level factors and one two\u2010level factor). The area under the receiver operating characteristics (ROC) curve was 0.966, showing the high accuracy of the test discriminating positive from negative samples. Taking into account the biosafety benefits, the high yields of antigen in cell culture, and the general performance of the assay, it is expected that it will be a useful alternative to the current ELISA for the detection of antibodies in sera from convalescent patients. J. Med. Virol. 80:2127\u20132133, 2008. \u00a9 2008 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jmv.21265","type":"journal-article","created":{"date-parts":[[2008,10,21]],"date-time":"2008-10-21T13:54:44Z","timestamp":1224597284000},"page":"2127-2133","source":"Crossref","is-referenced-by-count":9,"title":["Argentine hemorrhagic fever diagnostic test based on recombinant Jun\u00edn virus N protein"],"prefix":"10.1002","volume":"80","author":[{"given":"Agust\u00edn E.","family":"Ure","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P. Daniel","family":"Ghiringhelli","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robert D.","family":"Possee","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shigeru","family":"Morikawa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"V\u00edctor","family":"Romanowski","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2008,10,21]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1089\/vim.2006.19.196"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1119(87)90166-1"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1099\/0022-1317-71-1-19"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.2007.77.732"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1000047"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.antiviral.2007.10.010"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1099\/0022-1317-72-9-2129"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/s004300100061"},{"key":"e_1_2_1_10_1","first-page":"493","article-title":"Enzyme\u2010linked immunosorbent assay for detection of antibody to lymphocytic choriomeningitis virus in mouse sera, with recombinant nucleoprotein as antigen","volume":"45","author":"Homberger FR","year":"1995","journal-title":"Lab Anim Sci"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-1759(98)00185-9"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.virol.2007.02.008"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(89)91833-3"},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/0168-1702(93)90111-Y"},{"key":"e_1_2_1_15_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.33.5.1327-1332.1995"},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.1890400308"},{"key":"e_1_2_1_17_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02122437"},{"key":"e_1_2_1_18_1","doi-asserted-by":"publisher","DOI":"10.1099\/0022-1317-67-8-1515"},{"key":"e_1_2_1_19_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-642-56029-3_6"},{"key":"e_1_2_1_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0166-0934(01)00452-9"},{"key":"e_1_2_1_21_1","doi-asserted-by":"publisher","DOI":"10.1373\/clinchem.2004.031823"},{"key":"e_1_2_1_22_1","volume-title":"Baculovirus expression vectors: A laboratory manual","author":"O'Reilly DR","year":"1992"},{"key":"e_1_2_1_23_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa073785"},{"key":"e_1_2_1_24_1","first-page":"305","article-title":"Evaluation of an enzyme immunosorbent assay for the diagnosis of Argentine haemorrhagic fever","volume":"41","author":"Riera LM","year":"1997","journal-title":"Acta Virol"},{"key":"e_1_2_1_25_1","doi-asserted-by":"publisher","DOI":"10.1128\/CVI.00101-07"},{"key":"e_1_2_1_26_1","first-page":"725","volume-title":"Virus Taxonomy VIIIth report of the ICTV","author":"Salvato MS","year":"2005"},{"key":"e_1_2_1_27_1","volume-title":"Molecular cloning. A laboratory manual","author":"Sambrook J","year":"1989"},{"key":"e_1_2_1_28_1","doi-asserted-by":"publisher","DOI":"10.1099\/0022-1317-70-5-1125"},{"key":"e_1_2_1_29_1","volume-title":"Taguchi methods","author":"Taguchi G","year":"1993"},{"key":"e_1_2_1_30_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.36.11.3143-3148.1998"},{"key":"e_1_2_1_31_1","doi-asserted-by":"publisher","DOI":"10.1093\/clinchem\/39.4.561"}],"container-title":["Journal of Medical Virology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjmv.21265","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jmv.21265","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,15]],"date-time":"2023-11-15T00:28:53Z","timestamp":1700008133000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jmv.21265"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2008,10,21]]},"references-count":30,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2008,12]]}},"alternative-id":["10.1002\/jmv.21265"],"URL":"https:\/\/doi.org\/10.1002\/jmv.21265","archive":["Portico"],"relation":{},"ISSN":["0146-6615","1096-9071"],"issn-type":[{"value":"0146-6615","type":"print"},{"value":"1096-9071","type":"electronic"}],"subject":[],"published":{"date-parts":[[2008,10,21]]}}}