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Serological testing for dengue IgM was performed in Pakistan and polymerase chain reaction (PCR) testing for dengue RNA detection and serotyping were performed in Hong Kong. Dengue\u2010specific IgM was detectable as early as 1 day, and dengue RNA remained detectable for up to 14 days, post\u2010onset of illness. Further statistical analysis found that IgM status (positive, negative, or equivocal) was significantly correlated to clinical (duration of illness, severity of patient\u2010reported arthralgia pain, the presence of any evidence of bleeding, a positive tourniquet test, shock), and other laboratory (platelet and total white cell counts) parameters. In contrast, the qualitative dengue RNA status (PCR positive or negative) was not statistically significantly correlated with any of these other parameters. The results for this population during this outbreak, obtained from single acute samples, demonstrate a wide range of intervals post\u2010onset of illness during which dengue IgM and dengue RNA may be detected. Interestingly, in this study, the dengue IgM positivity correlates more closely with significant clinical illness than the dengue RNA positivity, which may be a feature specific to this particular outbreak. J. Med. Virol. 80:2113\u20132121, 2008. \u00a9 2008 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jmv.21290","type":"journal-article","created":{"date-parts":[[2008,10,21]],"date-time":"2008-10-21T13:54:44Z","timestamp":1224597284000},"page":"2113-2121","source":"Crossref","is-referenced-by-count":10,"title":["A wide spectrum of dengue IgM and PCR positivity post\u2010onset of illness found in a large dengue 3 outbreak in Pakistan"],"prefix":"10.1002","volume":"80","author":[{"given":"Julian W.","family":"Tang","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Muhammad R.","family":"Khanani","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Adnan M.","family":"Zubairi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wai 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