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They were investigated for the presence of enteroviruses using shell vial culture and indirect immunofluorescence assays. Most cases (46.9%) occurred in children younger than 1 year of age in which males represented 71.9%. The common symptoms were fever, vomiting, and headache. Enteroviruses were isolated from 32 (8.2%) cases, coxsackievirus B types 2, 4, and 5 from 15 (46.9%) cases, and echovirus 9 (31.3%) was the most common identified serotype. The virus isolation rate was directly proportional to the number of leukocytes in the cerebrospinal fluid. However, enteroviral isolation was demonstrated in 4 (12.5%) of 32 cerebrospinal fluid specimens without pleocytosis. Leukocyte differential count revealed a predominance of polymorphonuclear cells in 71.4% of the cases. Hospitalization ranged from 1 day to 25 days with a mean of 7 days. The majority of enterovirus\u2010infected patients (88.9%) were treated with at least one type of antibiotic. These results emphasize the importance of shell vial culture assay for diagnosing enteroviruses, especially in laboratories that do not have access to advanced techniques such as polymerase chain reaction. J. Med. Virol. 66:224\u2013228, 2002. \u00a9 2002 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jmv.2133","type":"journal-article","created":{"date-parts":[[2002,8,25]],"date-time":"2002-08-25T16:30:43Z","timestamp":1030293043000},"page":"224-228","source":"Crossref","is-referenced-by-count":23,"title":["Enteroviral meningitis in Northern Jordan: Prevalence and association with clinical findings"],"prefix":"10.1002","volume":"66","author":[{"given":"Mamdoh M.","family":"Meqdam","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mohamed M.","family":"Khalousi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Abdullah","family":"Al\u2010Shurman","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2001,12,13]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.2739\/kurumemedj.46.97"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0950268800059185"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1136\/adc.78.4.373"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1093\/tropej\/35.5.211-a"},{"key":"e_1_2_1_6_1","unstructured":"BaronS.1991.Medical Microbiology. 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