{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,5]],"date-time":"2026-04-05T02:58:01Z","timestamp":1775357881017,"version":"3.50.1"},"reference-count":34,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2008,12,23]],"date-time":"2008-12-23T00:00:00Z","timestamp":1229990400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2009,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Chromosomal integration of <jats:italic>human herpesvirus 6<\/jats:italic> (HHV\u20106) is a novel situation found in a small percentage of individuals. While active HHV\u20106 infection is treatable using antivirals, the abnormally high level of HHV\u20106 DNA found in chromosomal integration of HHV\u20106 (CI\u2010HHV\u20106) is not affected by such drugs. Stored DNA samples taken originally for detection of fusion genes and minimal residual disease from 339 pediatric patients treated for leukemia in the Czech Republic between the years 1995\u20132007 were tested retrospectively. Using real\u2010time quantitative PCR technology, the quantity of HHV\u20106 DNA detected was normalized to 100,000 human genome equivalents as assessed by quantitation of the albumin gene. HHV\u20106 DNA was detected in 107 samples from 91 patients (26.8%). In the majority of samples (99) only a minute level of normalized viral copies (NVCs) (median 1.84 NVCs) was detected. A high viral load of approximately 100,000 NVCs was detected in 5 patients (1.5%; median 140,150 NVCs), in all of whom were confirmed subsequently CI\u2010HHV\u20106 by a detection of HHV\u20106 DNA in hair follicles or in the nails. In all but one patient with HHV\u20106 variant B, variant A of the virus was detected. None of the patients with CI\u2010HHV\u20106 had complications attributable to HHV\u20106 infection. The prevalence of CI\u2010HHV\u20106 in childhood leukemia does not differ from that published for other patients or healthy populations. Where high levels of HHV\u20106 DNA are present, CI\u2010HHV\u20106 should be confirmed as soon as possible so that potentially toxic but ineffective antiviral treatment can be stopped. J. Med. Virol. 81:258\u2013263, 2009. \u00a9 2008 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jmv.21371","type":"journal-article","created":{"date-parts":[[2008,12,23]],"date-time":"2008-12-23T22:40:12Z","timestamp":1230072012000},"page":"258-263","source":"Crossref","is-referenced-by-count":45,"title":["Prevalence of HHV\u20106 integrated chromosomally among children treated for acute lymphoblastic or myeloid leukemia in the Czech Republic"],"prefix":"10.1002","volume":"81","author":[{"given":"Petr","family":"Hubacek","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Katerina","family":"Muzikova","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alena","family":"Hrdlickova","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ondrej","family":"Cinek","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Katerina","family":"Hyncicova","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hana","family":"Hrstkova","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Petr","family":"Sedlacek","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jan","family":"Stary","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2008,12,23]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1136\/adc.67.12.1484"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bmt.1702065"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1086\/322691"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.3324\/haematol.11638"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1086\/500838"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1086\/506953"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2141.1998.00903.x"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V94.5.1545"},{"key":"e_1_2_1_10_1","first-page":"1108","article-title":"Presence of human herpesvirus 6 DNAin somatic cells","volume":"95","author":"Daibata M","year":"2000","journal-title":"Blood"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.18.1.217-245.2005"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0166-0934(01)00390-1"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bmt.1705813"},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.3324\/haematol.11592"},{"key":"e_1_2_1_15_1","doi-asserted-by":"publisher","DOI":"10.1007\/s005350200081"},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-8749.1994.tb11903.x"},{"key":"e_1_2_1_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.20760"},{"key":"e_1_2_1_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2141.2000.02422.x"},{"key":"e_1_2_1_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.1890400110"},{"key":"e_1_2_1_20_1","first-page":"458","article-title":"Human herpesvirus 6 in hematologic diseases in China","volume":"85","author":"Ma XT","year":"2000","journal-title":"Haematologica"},{"key":"e_1_2_1_21_1","doi-asserted-by":"publisher","DOI":"10.1086\/317651"},{"key":"e_1_2_1_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/0387-7604(95)00077-O"},{"key":"e_1_2_1_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.leu.2401246"},{"key":"e_1_2_1_24_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V100.6.2005"},{"key":"e_1_2_1_25_1","doi-asserted-by":"publisher","DOI":"10.1002\/ana.410330607"},{"key":"e_1_2_1_26_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.20113"},{"key":"e_1_2_1_27_1","doi-asserted-by":"crossref","first-page":"4015","DOI":"10.1182\/blood.V87.9.4015.bloodjournal8794015","article-title":"Utility of fingernail DNA for evaluation of chimerism after bone marrow transplantation and for diagnostic testing for transfusion\u2010associated graft\u2010versus\u2010host disease","volume":"87","author":"Uchida S","year":"1996","journal-title":"Blood"},{"key":"e_1_2_1_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcv.2004.11.008"},{"key":"e_1_2_1_29_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.44.4.1571-1574.2006"},{"key":"e_1_2_1_30_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.02115-06"},{"key":"e_1_2_1_31_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.TP.0000119603.59880.47"},{"key":"e_1_2_1_32_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2141.2003.04788.x"},{"key":"e_1_2_1_33_1","doi-asserted-by":"publisher","DOI":"10.1097\/00006454-199305000-00014"},{"key":"e_1_2_1_34_1","doi-asserted-by":"publisher","DOI":"10.1136\/jcp.54.10.792"},{"key":"e_1_2_1_35_1","doi-asserted-by":"publisher","DOI":"10.1086\/428060"}],"container-title":["Journal of Medical Virology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjmv.21371","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jmv.21371","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,13]],"date-time":"2023-11-13T21:23:47Z","timestamp":1699910627000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jmv.21371"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2008,12,23]]},"references-count":34,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2009,2]]}},"alternative-id":["10.1002\/jmv.21371"],"URL":"https:\/\/doi.org\/10.1002\/jmv.21371","archive":["Portico"],"relation":{},"ISSN":["0146-6615","1096-9071"],"issn-type":[{"value":"0146-6615","type":"print"},{"value":"1096-9071","type":"electronic"}],"subject":[],"published":{"date-parts":[[2008,12,23]]}}}