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For further characterization of islet cell interactions with a passaged virus strain, a murine insulinoma cell line, MIN\u20106, was selected as an experimental model. The CBV\u20105\u2010MPP virus strain was not able to replicate in MIN\u20106 cells in vitro but required adaptation over a few days for progeny production and the generation of cytopathic effects. In order to determine the genetic characteristics required for virus growth in MIN\u20106 cells, the whole genome of the MIN\u20106\u2010adapted virus variant was sequenced, and critical amino acids were identified by comparing the sequence with that of a virus strain passaged repeatedly in the mouse pancreas. The results of site\u2010directed mutagenesis demonstrated that only one residue, amino acid 94 of VP1, is a major determinant for virus adaptation to MIN\u20106 cells. J. Med. Virol. 81:296\u2013304, 2009. \u00a9 2008 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jmv.21391","type":"journal-article","created":{"date-parts":[[2008,12,23]],"date-time":"2008-12-23T22:40:12Z","timestamp":1230072012000},"page":"296-304","source":"Crossref","is-referenced-by-count":17,"title":["Amino acids of coxsackie B5 virus are critical for infection of the murine insulinoma cell line, 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