{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,13]],"date-time":"2026-03-13T06:16:45Z","timestamp":1773382605273,"version":"3.50.1"},"reference-count":39,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2009,1,16]],"date-time":"2009-01-16T00:00:00Z","timestamp":1232064000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2009,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>In order to prevent cervical cancer, vaccines against human papilloma virus types 16 (HPV\u201016) and 18 (HPV\u201018) have been implemented worldwide. However, the HPV types that cause cancer can differ according to geographical area and ethnicity. In this new era of the HPV vaccine, it is important to elucidate the prevalent HPV types in each area. Therefore, the prevalence of HPV infection and cervical abnormalities among 369 female commercial sex workers in the Philippines were examined. HPV <jats:italic>L1<\/jats:italic> gene was amplified by polymerase chain reaction (PCR) using modified GP5+\/6+ primers, and genotyping was performed by sequencing cloned PCR products. HPV DNA was detected in 211 (57.2%) women, among whom 46 HPV types were identified. HPV\u201052 was most common and multiple\u2010type infection was observed in 44.5%. Among 56 women with abnormal cervical cytology (low\u2010 and high\u2010grade squamous intraepithelial lesions and adenocarcinoma in situ), HPV\u201052 was most common (23.2%), followed by HPV\u201016 (19.6%), \u201058 (10.7%), and \u201067 (10.7%). Only 27% of these women were positive for HPV\u201016 and \u201018. Multivariate analysis revealed that HPV\u201016, \u201039, \u201052, \u201067, and \u201082 were significantly associated with abnormal cytology. Repeated analysis of HPV\u201052 single\u2010positive samples using the original GP5+\/6+ PCR primers produced negative results in 57% of cases, suggesting that the prevalence of HPV\u201052 infection may have been underestimated in previous studies, and the current vaccines may not be sufficient for preventing infection and the development of premalignant lesions of the cervix in women in the Philippines. J. Med. Virol. 81:545\u2013551, 2009. \u00a9 2009 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jmv.21416","type":"journal-article","created":{"date-parts":[[2009,1,17]],"date-time":"2009-01-17T01:58:33Z","timestamp":1232157513000},"page":"545-551","source":"Crossref","is-referenced-by-count":23,"title":["High\u2010risk HPV types in lesions of the uterine cervix of female commercial sex workers in the Philippines"],"prefix":"10.1002","volume":"81","author":[{"given":"Michiko","family":"Miyashita","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dorothy 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