{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,2,15]],"date-time":"2024-02-15T14:10:36Z","timestamp":1708006236464},"reference-count":38,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2009,1,16]],"date-time":"2009-01-16T00:00:00Z","timestamp":1232064000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2009,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The development of HTLV\u20101 associated clinical manifestations, such as TSP\/HAM and ATLL, occur in 2\u20134% of the infected population and it is still unclear why this infection remains asymptomatic in most infected carriers. Recently, it has been demonstrated that HTLV uses the Glucose transporter type 1 (GLUT1) to infect T\u2010CD4<jats:sup>+<\/jats:sup> lymphocytes and that single nucleotide polymorphisms (SNP) in the <jats:italic>GLUT1<\/jats:italic> gene are associated with diabetic nephropathy in patients with diabetes mellitus in different populations. These polymorphisms could contribute to a higher GLUT1 protein expression on cellular membrane, facilitating the entry of HTLV and its transmission cell by cell. This could result in a higher provirus load and consequently in the development of TSP\/HAM. To evaluate the role of <jats:italic>GLUT1<\/jats:italic> gene polymorphisms in the development of TSP\/HAM in HTLV\u20101 infected individuals, the g.22999G\u2009&gt;\u2009T, g.15339T\u2009&gt;\u2009C and c.\u20102841A\u2009&gt;\u2009T sites were analyzed by PCR\/RFLP or sequencing in 244 infected individuals and 102 normal controls. The proviral load of the HTLV\u20101 infected patients was also analyzed using Real Time Quantitative PCR. Genotypic and allelic frequencies of the three sites did not differ significantly between controls and HTLV\u20101 infected individuals. There was no difference in genotypic and allelic distributions among patients as to the presence or absence of HTLV\u20101 associated clinic manifestations. As regards the quantification of the provirus load, we observed a significant reduction in the asymptomatic individuals compared with the oligosymptomatic and TSP\/HAM individuals. These results suggest that g.22999G\u2009&gt;\u2009T, g.15339T\u2009&gt;\u2009C, and c.\u20102841A\u2009&gt;\u2009T SNP do not contribute to HTLV\u20101 infection nor to the genetic susceptibility of TSP\/HAM in Brazilian HTLV\u20101 infected individuals. J. Med. Virol. 81:552\u2013557, 2009. \u00a9 2009 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jmv.21421","type":"journal-article","created":{"date-parts":[[2009,1,17]],"date-time":"2009-01-17T01:58:33Z","timestamp":1232157513000},"page":"552-557","source":"Crossref","is-referenced-by-count":4,"title":["Polymorphisms at <i>GLUT1<\/i> gene are not associated with the development of TSP\/HAM in Brazilian HTLV\u20101 infected individuals and the discovery of a new polymorphism at <i>GLUT1<\/i> gene"],"prefix":"10.1002","volume":"81","author":[{"given":"Giselle Calasans Souza","family":"Costa","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rochele","family":"Azevedo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sandra Rocha","family":"Gadelha","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Simone Haddad","family":"Kashima","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gabriel","family":"Muricy","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Viviana Nila","family":"Olavarria","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dimas Tadeu","family":"Covas","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Osvaldo Massaiti","family":"Takayanagui","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bernardo","family":"Galv\u00e3o\u2010Castro","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luiz Carlos J\u00fanior","family":"Alcantara","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2009,1,16]]},"reference":[{"key":"e_1_2_1_2_1","volume-title":"BioEstat. Vers\u00e3o 4.0, Sociedade Civil Mamirau\u00e1","author":"Ayres M","year":"2005"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1080115"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.79.7.4150-4158.2005"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1097\/00007890-200108270-00027"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cyto.2004.06.005"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0166-0934(01)00445-1"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1097\/00126334-200312150-00013"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI2629"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1353\/hub.2005.0061"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.21341"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(85)92734-5"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1523-1755.2001.059002631.x"},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0168-8227(98)00050-3"},{"key":"e_1_2_1_15_1","doi-asserted-by":"publisher","DOI":"10.1677\/joe.1.05599"},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jdiacomp.2004.07.002"},{"key":"e_1_2_1_17_1","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-6143.2002.20611.x"},{"key":"e_1_2_1_18_1","doi-asserted-by":"publisher","DOI":"10.1097\/00042560-199606010-00014"},{"key":"e_1_2_1_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/anr.1780321030"},{"key":"e_1_2_1_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/0140-6736(90)92896-P"},{"key":"e_1_2_1_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0197-4580(03)00013-7"},{"key":"e_1_2_1_22_1","first-page":"662","article-title":"Insulin receptor substrate\u20101 and glucose transporter gene polymorphisms in noninsulin\u2010dependent diabetes mellitus","volume":"78","author":"Liu Z","year":"1998","journal-title":"Zhonghua Yi Xue Za Zhi"},{"key":"e_1_2_1_23_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1523-1755.1999.00449.x"},{"key":"e_1_2_1_24_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M504549200"},{"key":"e_1_2_1_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/0140-6736(92)90699-4"},{"key":"e_1_2_1_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(89)91793-5"},{"key":"e_1_2_1_27_1","doi-asserted-by":"publisher","DOI":"10.3109\/13550289809114225"},{"key":"e_1_2_1_28_1","doi-asserted-by":"publisher","DOI":"10.2337\/diabetes.51.7.2264"},{"key":"e_1_2_1_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0198-8859(02)00419-6"},{"key":"e_1_2_1_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(86)91298-5"},{"key":"e_1_2_1_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.transproceed.2003.10.013"},{"key":"e_1_2_1_32_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.77.12.7415"},{"key":"e_1_2_1_33_1","doi-asserted-by":"publisher","DOI":"10.1093\/oxfordjournals.jhered.a111573"},{"key":"e_1_2_1_34_1","doi-asserted-by":"publisher","DOI":"10.1164\/ajrccm.156.4.12tac-14"},{"key":"e_1_2_1_35_1","doi-asserted-by":"publisher","DOI":"10.1086\/423942"},{"key":"e_1_2_1_36_1","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1080\/13550280390173418","article-title":"Usefulness of proviral load measurement for monitoring of disease activity in individual patients with human T\u2010lymphotropic virus type 1\u2010associated myelopathy\/tropical spastic paraparesis","volume":"9","author":"Takenouchi N","year":"2003","journal-title":"J Neurovirol"},{"key":"e_1_2_1_37_1","doi-asserted-by":"publisher","DOI":"10.1042\/BJ20070286"},{"key":"e_1_2_1_38_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.79.6.2031"},{"key":"e_1_2_1_39_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1464-5491.2002.00844.x"}],"container-title":["Journal of Medical Virology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjmv.21421","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jmv.21421","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,13]],"date-time":"2023-11-13T17:24:14Z","timestamp":1699896254000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jmv.21421"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2009,1,16]]},"references-count":38,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2009,3]]}},"alternative-id":["10.1002\/jmv.21421"],"URL":"https:\/\/doi.org\/10.1002\/jmv.21421","archive":["Portico"],"relation":{},"ISSN":["0146-6615","1096-9071"],"issn-type":[{"value":"0146-6615","type":"print"},{"value":"1096-9071","type":"electronic"}],"subject":[],"published":{"date-parts":[[2009,1,16]]}}}