{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,18]],"date-time":"2023-10-18T11:16:29Z","timestamp":1697627789257},"reference-count":19,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2009,4,20]],"date-time":"2009-04-20T00:00:00Z","timestamp":1240185600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2009,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Following infection with hepatitis E virus (HEV), anti\u2010HEV immunoglobulin (Ig) M is thought to develop before anti\u2010HEV IgG and to be a better marker for differentiating between the acute and convalescent phases of infection. In order to select polypeptides for improved detection of anti\u2010HEV IgM, six and three overlapping polypeptides from open reading frames (ORFs) 2 and 3, respectively, of HEV genotypes 1 and 4 were expressed as fusion proteins in <jats:italic>Escherichia coli<\/jats:italic>. The reactivities of the polypeptides with anti\u2010HEV IgM were evaluated using immunoblotting and enzyme immunoassays (EIAs). The data indicated that polypeptides from the N\u2010terminus of ORF3 and middle region of ORF2 were weakly or not reactive with anti\u2010HEV IgM, while those from the remaining regions of ORF2 and ORF3 contained reactive epitopes. Anti\u2010HEV IgM against the N\u2010 or C\u2010terminus of ORF2 appeared earlier and disappeared faster than that against polypeptides from the C\u2010terminus of ORF3, based on serum samples from rhesus monkeys infected experimentally, and from patients infected naturally, with HEV. The N\u2010 and C\u2010terminal polypeptides from ORF2 complemented one another in detecting anti\u2010HEV IgM and EIA sensitivity was improved significantly with a combination of these polypeptides. The reactivities of ORF2 polypeptides from genotypes 1 and 4 were similar but that of ORF3 differed with sera from monkeys infected by the two genotypes. Thus, a combination of N\u2010 and C\u2010terminal polypeptides of ORF2 from one genotype may be effective in EIAs to detect anti\u2010HEV IgM. J. Med. Virol. 81:1052\u20131061, 2009. \u00a9 2009 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jmv.21484","type":"journal-article","created":{"date-parts":[[2009,4,21]],"date-time":"2009-04-21T05:18:47Z","timestamp":1240291127000},"page":"1052-1061","source":"Crossref","is-referenced-by-count":25,"title":["Varying abilities of recombinant polypeptides from different regions of hepatitis E virus ORF2 and ORF3 to detect anti\u2010HEV immunoglobulin M"],"prefix":"10.1002","volume":"81","author":[{"given":"Hongxia","family":"Ma","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaoguo","family":"Song","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhuo","family":"Li","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tim 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