{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,26]],"date-time":"2026-10-26T03:15:09Z","timestamp":1792984509492,"version":"4.5.0"},"reference-count":25,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2009,4,20]],"date-time":"2009-04-20T00:00:00Z","timestamp":1240185600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2009,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Monkeypox virus (MPXV) causes a smallpox\u2010like disease in non\u2010human primates and humans. This infection is endemic to central and western Africa. MPXV is divided into two genetically different groups, Congo Basin and West African MPXV, with the former being the more virulent. A real\u2010time quantitative MPXV genome amplification system was developed for the diagnosis of MPXV infections using loop\u2010mediated isothermal amplification (LAMP) technology. Primers used for genome amplification of Congo Basin (C\u2010LAMP), West African (W\u2010LAMP), and both Congo Basin and West African (COM\u2010LAMP) MPXV by LAMP were designed according to the nucleotide sequences of the Congo Basin\u2010specific D14L gene, the West African\u2010specific partial ATI gene, and the partial ATI gene that is shared by both groups, respectively. The sensitivity and specificity of the LAMP were evaluated with nested PCR using peripheral blood and throat swab specimens collected from Congo Basin MPXV or West African MPXV\u2010infected monkeys. The sensitivity and specificity of COM\u2010LAMP, C\u2010LAMP, and W\u2010LAMP were 80% (45\/56) and 100% (64\/64); 79% (19\/24) and 100% (24\/24); and 72% (23\/32) and 100% (40\/40), respectively. The viremia level determined by LAMP assays increased with increases in the severity of the monkeypox\u2010associated symptoms. The newly developed LAMP assay was confirmed to be a rapid, quantifiable, and highly sensitive and specific system effective in the diagnosis of MPXV infections. The LAMP assays made it possible to discriminate between Congo Basin and West African MPXV. The LAMP developed in this study is useful not only for diagnosis of but also for the assessment of MPXV infections. J. Med. Virol. 81:1102\u20131108, 2009. \u00a9 2009 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jmv.21494","type":"journal-article","created":{"date-parts":[[2009,4,21]],"date-time":"2009-04-21T05:18:47Z","timestamp":1240291127000},"page":"1102-1108","source":"Crossref","is-referenced-by-count":94,"title":["Loop\u2010mediated isothermal amplification\u2010based diagnostic assay for monkeypox virus 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