{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T15:26:08Z","timestamp":1753889168790},"reference-count":35,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2009,7,22]],"date-time":"2009-07-22T00:00:00Z","timestamp":1248220800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2009,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>HCV replicates in liver via an intermediate negative strand RNA. To study the relevance of HCV genome replication, quantitative strand\u2010specific HCV real\u2010time RT\u2010PCR assays were developed and applied to livers explanted because of end\u2010stage cirrhosis. The assays have broad ranges of determination and a high reproducibility and accuracy. Analysis of five different samples showed an even distribution of HCV genomes in four livers. Hepatic concentrations of positive (PS)\u2010 and negative (NS)\u2010strand RNA did correlate with each other, with PS\/NS ratios ranging between 3 and 340. Hepatic concentrations of HCV\u2010PS or \u2010NS RNA did not correlate with serum HCV\u2010RNA levels or with genotypes. A high HCV envelope\u20102 protein expression correlated with a low NS concentration. HCV\u2010PS and \u2010NS levels, E2 protein expression and genotype did not correlate with biochemical tests or with histological changes in the explanted liver, but the ratio NS\/PS, a marker of viral replication, correlated with the severity of the recurrent post\u2010transplant hepatitis caused by HCV. This suggests the existence of an extra\u2010hepatic location of HCV with comparable viral replication rate being responsible for the infection of the newly transplanted liver. J. Med. Virol. 81:1569\u20131575, 2009. \u00a9 2009 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jmv.21510","type":"journal-article","created":{"date-parts":[[2009,7,22]],"date-time":"2009-07-22T19:03:21Z","timestamp":1248289401000},"page":"1569-1575","source":"Crossref","is-referenced-by-count":6,"title":["Quantitation of replication of the HCV genome in human livers with end\u2010stage cirrhosis by strand\u2010specific real\u2010time RT\u2010PCR assays: Methods and clinical 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