{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,12,24]],"date-time":"2023-12-24T15:10:12Z","timestamp":1703430612794},"reference-count":61,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2009,11,30]],"date-time":"2009-11-30T00:00:00Z","timestamp":1259539200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2010,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Hemorrhagic cystitis is characterized by hematuria due to inflammation of the bladder. In bone marrow transplants, this disease is linked to the infection by human polyomavirus BK, whereas the role of the human polyomavirus JC is unclear. The transcriptional control regions of both viruses contain important cellular transcription factor binding sites that undergo rearrangement process generating suitable variants that could be more active for viral replication and for the onset of hemorrhagic cystitis. In this study urine obtained from seven patients with bone marrow transplant were examined. Polyomavirus genomes were quantified by PCR and viral loads were compared. The transcriptional regions of both viruses were amplified and sequenced to determine the presence of variants. Subtypes of polyomaviruses were determined by amplification and sequencing of the viral protein 1 region. The results showed that four of seven patients were positive for BK DNA, two of seven patients had BK and JC DNA and one of seven had JC DNA. Positive samples were amplified and sequenced successively for transcriptional regions. The viral archetype was always found in both viruses. Finally, typing showed that BK virus subtype I infected patients with BK, whereas JC virus genotype IA and genotype 1B were found in patients infected with JC. The data suggest that new and different approaches are required to improve the morbidity and mortality caused by polyoma\u2010associated hemorrhagic cystitis, since it known that BK virus is involved in the onset of hemorrhagic cystitis, whereas the role of JC virus should be investigated further. J. Med. Virol. 82:138\u2013145, 2010. \u00a9 2009 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jmv.21558","type":"journal-article","created":{"date-parts":[[2009,11,30]],"date-time":"2009-11-30T20:04:41Z","timestamp":1259611481000},"page":"138-145","source":"Crossref","is-referenced-by-count":9,"title":["Viral infection in bone marrow transplants: Is JC virus involved?"],"prefix":"10.1002","volume":"82","author":[{"given":"Monica","family":"Mischitelli","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daniela","family":"Fioriti","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elena","family":"Anzivino","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anna","family":"Bellizzi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Valentina","family":"Barucca","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Renzo","family":"Boldorini","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Umberto","family":"Miglio","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Simona","family":"Sica","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Federica","family":"Sor\u00e0","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Silvia","family":"De Matteis","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fernanda","family":"Chiarini","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Valeria","family":"Pietropaolo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2009,11,30]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1099\/0022-1317-82-5-1221"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/0471221945.ch18"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/0-387-32957-9_1"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1386-6532(99)00055-4"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.1995.13.5.1103"},{"key":"e_1_2_1_7_1","first-page":"573","article-title":"Presence of human polyomavirus DNA in the peripheral circulation of bone marrow transplant patients with and without haemorrhagic cystitis","volume":"17","author":"Bogdanovic G","year":"1996","journal-title":"Bone Marrow Transplant"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.42.11.5394-5396.2004"},{"key":"e_1_2_1_9_1","first-page":"288","article-title":"Primary BK virus (BKV) infection due to possible BKV transmission during bone marrow transplantation is not the major cause of haemorrhagic cystitis in transplanted children","volume":"2","author":"Bogdanovic G","year":"1998","journal-title":"Pediatr Transplant"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1080\/00313020701716458"},{"key":"e_1_2_1_11_1","doi-asserted-by":"crossref","first-page":"766","DOI":"10.5858\/133.5.766","article-title":"Detection, distribution and pathological significance of BK virus strains isolated from kidney transplant patients with and without polyomavirus\u2010associated nephropathy","volume":"133","author":"Boldorini R","year":"2009","journal-title":"Arch Pathol Lab Med"},{"key":"e_1_2_1_12_1","first-page":"24","article-title":"Post\u2010transplantation polyomavirus infections","volume":"12","author":"Boubenider S","year":"1999","journal-title":"J Nephrol"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF01311159"},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.1989.7.2.194"},{"key":"e_1_2_1_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.20566"},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-5347(17)39848-8"},{"key":"e_1_2_1_17_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.tp.0000269706.59977.a5"},{"key":"e_1_2_1_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bmt.1705886"},{"key":"e_1_2_1_19_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2004-12-4988"},{"key":"e_1_2_1_20_1","doi-asserted-by":"publisher","DOI":"10.1080\/713831413"},{"key":"e_1_2_1_21_1","doi-asserted-by":"publisher","DOI":"10.1177\/039463200501800213"},{"key":"e_1_2_1_22_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.51.2.458-469.1984"},{"key":"e_1_2_1_23_1","first-page":"401","article-title":"The incidence of haemorrhagic cystitis and BK\u2010viruria in allogeneic hematopoietic stem cell recipients according to intensity of the conditioning regimen","volume":"91","author":"Giraud G","year":"2006","journal-title":"Haematologica"},{"key":"e_1_2_1_24_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bmt.1705962"},{"key":"e_1_2_1_25_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20072097"},{"key":"e_1_2_1_26_1","doi-asserted-by":"publisher","DOI":"10.1099\/vir.0.82266-0"},{"key":"e_1_2_1_27_1","doi-asserted-by":"publisher","DOI":"10.1006\/viro.1999.0092"},{"key":"e_1_2_1_28_1","doi-asserted-by":"publisher","DOI":"10.1002\/0471221945.ch5"},{"key":"e_1_2_1_29_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.1890410104"},{"key":"e_1_2_1_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0928-0197(94)00044-1"},{"key":"e_1_2_1_31_1","doi-asserted-by":"publisher","DOI":"10.1099\/0022-1317-79-10-2491"},{"key":"e_1_2_1_32_1","doi-asserted-by":"publisher","DOI":"10.1007\/s007050170023"},{"key":"e_1_2_1_33_1","doi-asserted-by":"publisher","DOI":"10.1007\/0-387-32957-9_20"},{"key":"e_1_2_1_34_1","doi-asserted-by":"publisher","DOI":"10.1002\/0471221945.ch6"},{"key":"e_1_2_1_35_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.21153"},{"key":"e_1_2_1_36_1","doi-asserted-by":"publisher","DOI":"10.1007\/0-387-32957-9_2"},{"key":"e_1_2_1_37_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11262-007-0166-0"},{"key":"e_1_2_1_38_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V98.6.1971"},{"key":"e_1_2_1_39_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bmt.1705139"},{"key":"e_1_2_1_40_1","first-page":"271","article-title":"BKV QPCR detection and infection monitoring in renal transplant recipients","volume":"30","author":"Mischitelli M","year":"2007","journal-title":"New Microbiol"},{"key":"e_1_2_1_41_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.20375"},{"key":"e_1_2_1_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.virol.2004.10.021"},{"key":"e_1_2_1_43_1","doi-asserted-by":"crossref","first-page":"559","DOI":"10.1080\/13550280390241269","article-title":"Molecular analysis of JC virus genotypes circulating among the Italian healthy population","volume":"9","author":"Pagani E","year":"2003","journal-title":"J Neurovirol"},{"key":"e_1_2_1_44_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1096-9071(199812)56:4<372::AID-JMV14>3.0.CO;2-4"},{"key":"e_1_2_1_45_1","doi-asserted-by":"publisher","DOI":"10.1080\/714044482"},{"key":"e_1_2_1_46_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bmt.1701010"},{"key":"e_1_2_1_47_1","doi-asserted-by":"publisher","DOI":"10.1007\/s15010-002-2010-8"},{"key":"e_1_2_1_48_1","doi-asserted-by":"publisher","DOI":"10.1086\/321813"},{"key":"e_1_2_1_49_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.20829"},{"key":"e_1_2_1_50_1","first-page":"533","article-title":"Haemorrhagic cystitis in pediatric bone marrow transplant patients: an association with infective agents, GVHD and prior cyclophosphamide","volume":"13","author":"Russel SJ","year":"1994","journal-title":"Bone Marrow Transplant"},{"key":"e_1_2_1_51_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM198312013092202"},{"key":"e_1_2_1_52_1","doi-asserted-by":"publisher","DOI":"10.1097\/00007890-199310000-00020"},{"key":"e_1_2_1_53_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.20909"},{"key":"e_1_2_1_54_1","doi-asserted-by":"publisher","DOI":"10.1002\/0471221945.ch22"},{"key":"e_1_2_1_55_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1286-4579(00)01339-3"},{"key":"e_1_2_1_56_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.32.5.1390-1394.1994"},{"key":"e_1_2_1_57_1","doi-asserted-by":"publisher","DOI":"10.3109\/13550280009018304"},{"key":"e_1_2_1_58_1","first-page":"1666","article-title":"Haemorrhagic cystitis following hematopoietic stem cell transplantation: incidence, risk factors and association with CMV reactivation and graft\u2010versus\u2010host disease","volume":"5","author":"Xu LP","year":"2007","journal-title":"Chin Med J"},{"key":"e_1_2_1_59_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0090-4295(94)80085-5"},{"key":"e_1_2_1_60_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1442-200X.2008.02549.x"},{"key":"e_1_2_1_61_1","doi-asserted-by":"publisher","DOI":"10.1002\/pros.10157"},{"key":"e_1_2_1_62_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.micinf.2006.11.008"}],"container-title":["Journal of Medical Virology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjmv.21558","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jmv.21558","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,22]],"date-time":"2023-11-22T15:41:09Z","timestamp":1700667669000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jmv.21558"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2009,11,30]]},"references-count":61,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2010,1]]}},"alternative-id":["10.1002\/jmv.21558"],"URL":"https:\/\/doi.org\/10.1002\/jmv.21558","archive":["Portico"],"relation":{},"ISSN":["0146-6615","1096-9071"],"issn-type":[{"value":"0146-6615","type":"print"},{"value":"1096-9071","type":"electronic"}],"subject":[],"published":{"date-parts":[[2009,11,30]]}}}