{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T23:41:49Z","timestamp":1777419709405,"version":"3.51.4"},"reference-count":17,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2002,1,4]],"date-time":"2002-01-04T00:00:00Z","timestamp":1010102400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2002,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>We previously isolated an acyclovir (ACV)\u2010resistant herpes simplex virus type 1 (HSV\u20101), strain TAR, from a child with Wiskott\u2010Aldrich syndrome. An acyclovir\u2010sensitive HSV\u20101, strain TAS, had been isolated from the same patient before the isolation of HSV\u20101 TAR. The TK protein of ACV\u2010sensitive HSV\u20101 TAS was composed of 376 amino acids, while that of HSV\u20101 TAR was composed of 407 amino acids with altered amino acid residue between positions 355\u2013407. The elongation of TK was caused by a single nucleotide deletion of cytosine from a homopolymer stretch of 4 cytosine residues between positions 1061\u20131064. There was no viral TK activity in HSV\u20101 TAR\u2010infected Vero cells, indicating the importance of the C\u2010terminal portion of TK protein from positions 355\u2013376. Recombinant TK polypeptides with amino acid deletions at the C\u2010terminus were prepared, and TK and ACV\u2010phosphorylation activities were examined. Deletion of 5 and 6 amino acids from the C\u2010terminus of the TK polypeptide of HSV\u20101 TAS resulted in a reduction of TK activity by approximately 75% and 100%, respectively. These mutant TK polypeptides did not phosphorylate ACV. These results indicate that amino acid residues from positions 371\u2013376 in the C\u2010terminal portion of HSV\u20101 TK protein are essential for keeping TK and ACV\u2010phosphorylation activities. J. Med. Virol. 66:388\u2010393, 2002. \u00a9 2002 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jmv.2157","type":"journal-article","created":{"date-parts":[[2002,8,25]],"date-time":"2002-08-25T16:33:59Z","timestamp":1030293239000},"page":"388-393","source":"Crossref","is-referenced-by-count":12,"title":["Importance of C\u2010terminus of herpes simplex virus type 1 thymidine kinase for maintaining thymidine kinase and acyclovir\u2010phosphorylation activities"],"prefix":"10.1002","volume":"66","author":[{"given":"Masayuki","family":"Saijo","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tatsuo","family":"Suzutani","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masahiro","family":"Niikura","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shigeru","family":"Morikawa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ichiro","family":"Kurane","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2002,1,4]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/0042-6822(91)90093-Q"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/0042-6822(89)90261-4"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1099\/0022-1317-79-9-2083"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1086\/515375"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1099\/0022-1317-77-8-1893"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1099\/0022-1317-24-3-465"},{"key":"e_1_2_1_8_1","doi-asserted-by":"crossref","first-page":"680","DOI":"10.1038\/227680a0","article-title":"Cleavage of structural proteins during the assembly of the head of bacteriophage T4","volume":"224","author":"Laemmli UK","year":"1970","journal-title":"Nature"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/0042-6822(88)90308-X"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1177\/095632029100200504"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/0168-1702(92)90105-I"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2133.1998.02374.x"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1096-9071(199908)58:4<387::AID-JMV11>3.0.CO;2-K"},{"key":"e_1_2_1_14_1","doi-asserted-by":"crossref","first-page":"3872","DOI":"10.1128\/jvi.71.5.3872-3878.1997","article-title":"Homopolymer mutational hot spots mediate herpes simplex virus resistance to acyclovir","volume":"71","author":"Sasadeusz JJ","year":"1997","journal-title":"J Virol"},{"key":"e_1_2_1_15_1","doi-asserted-by":"publisher","DOI":"10.1099\/0022-1317-69-10-2585"},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.1086\/314900"},{"key":"e_1_2_1_17_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.32.7.1046"},{"key":"e_1_2_1_18_1","doi-asserted-by":"publisher","DOI":"10.1007\/s007050050223"}],"container-title":["Journal of Medical Virology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjmv.2157","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jmv.2157","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,18]],"date-time":"2023-11-18T15:25:19Z","timestamp":1700321119000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jmv.2157"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2002,1,4]]},"references-count":17,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2002,3]]}},"alternative-id":["10.1002\/jmv.2157"],"URL":"https:\/\/doi.org\/10.1002\/jmv.2157","archive":["Portico"],"relation":{},"ISSN":["0146-6615","1096-9071"],"issn-type":[{"value":"0146-6615","type":"print"},{"value":"1096-9071","type":"electronic"}],"subject":[],"published":{"date-parts":[[2002,1,4]]}}}