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However, the mechanism of pathogenesis of HBV\u2010associated carcinoma remains elusive. In this study, the human hepatoblastoma HepG2 cell line harboring 1.2\u2009\u00d7\u2009unit\u2010length of the HBV genome was generated and subjected to a proteomic approach analyzing the global protein expression profiles of HepG2 cells with and without HBV replication and protein expression. By using fluorescence two\u2010dimensional difference gel electrophoresis (2D\u2010DIGE), followed by MALDI\u2010TOF\u2010MS and database searching, a total of 50 differentially expressed proteins were identified, including some cell cycle\u2010related proteins. These cycle\u2010related proteins may lead to accumulation of HepG2\u2010HBV cells in the G2\/M phase, and an increase in the proportion of HepG2 cells with tripolar or multipolar spindles. This study described the proteomic alterations in HepG2 cells HBV\u2010harboring, which may provide new insights into the underlying molecular mechanisms involved in HBV replication and pathogenesis. J. Med. Virol. 81:1539\u20131550, 2009. \u00a9 2009 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jmv.21593","type":"journal-article","created":{"date-parts":[[2009,7,22]],"date-time":"2009-07-22T19:03:37Z","timestamp":1248289417000},"page":"1539-1550","source":"Crossref","is-referenced-by-count":20,"title":["Global proteome analysis of hepatitis B virus expressing human hepatoblastoma cell line 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