{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,4,8]],"date-time":"2025-04-08T23:03:14Z","timestamp":1744153394812},"reference-count":35,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2009,12,22]],"date-time":"2009-12-22T00:00:00Z","timestamp":1261440000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2010,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Immunity to rubella virus (RV) is conventionally determined by measuring specific immunoglobulin G (IgG). However, several individuals may be considered immune despite undetectable antibody levels. In the present study RV\u2010specific interferon\u2010gamma (IFN\u03b3)\u2010ELISpot and rubella\u2010IgG\u2010ELISA were compared in 75 young adults aged between 20 and 30 years. In a subgroup, not only rubella\u2010like particles (RLP), but also HPV77 rubella vaccine derived antigen was used in IFN\u03b3\u2010ELISpot. The results from both, ELISA and ELISpot were independent of previous encounter to RV (vaccination, exanthematous disease, or childhood infection). There was no difference between RLP and RV vaccine antigen in IFN\u03b3\u2010ELISpot response, and there was no correlation between IFN\u03b3\u2010ELISpot and RV\u2010specific IgG levels. IFN\u03b3\u2010producing cells were found in 78.7% of all tested persons, and 83.8% of them were positive in ELISA. In almost all individuals seronegative for RV antibody, IFN\u03b3\u2010producing cells were detected. Considering both humoral and cell\u2010mediated immune responses, a positive RV immune reaction was seen in 98.6%. The results indicate that the IFN\u03b3\u2010ELISpot can provide valuable additional information in seronegative individuals. J. Med. Virol. 82:335\u2013340, 2010. \u00a9 2009 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jmv.21621","type":"journal-article","created":{"date-parts":[[2009,12,22]],"date-time":"2009-12-22T14:36:50Z","timestamp":1261492610000},"page":"335-340","source":"Crossref","is-referenced-by-count":6,"title":["Determination of rubella virus\u2010specific cell\u2010mediated immunity using 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