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Three published RT\u2010PCR primers sets targeting separately these three gene regions were used. Initially, from a total of 86 field isolates (36 HEV\u2010A, 40 HEV\u2010B, and 10 HEV\u2010C) tested, 100% concordance in HEV\u2010A was identified from all three gene regions (VP4, VP2, and VP1). However, for HEV\u2010B and HEV\u2010C viruses, only the VP2 and VP1 regions, and not VP4, showed 100% concordance in typing these viruses. To evaluate further the usefulness of VP4 in typing HEV\u2010A enteroviruses, 55 Japanese and 203 published paired VP4 and VP1 nucleotide sequences were also examined. In each case, typing by VP4 was 100% in concordance with typing using VP1. Given these results, it is proposed that for HEV\u2010A enteroviruses, all three gene regions (VP4, VP2, and VP1), would be useful for typing these viruses. These options would enhance the capability of laboratories in identifying these viruses and would greatly help in outbreaks of hand, foot, and mouth disease. J. 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Virol. 82:649\u2013657, 2010. \u00a9 2010 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jmv.21652","type":"journal-article","created":{"date-parts":[[2010,2,17]],"date-time":"2010-02-17T22:25:27Z","timestamp":1266445527000},"page":"649-657","source":"Crossref","is-referenced-by-count":28,"title":["A comparison of the VP1, VP2, and VP4 regions for molecular typing of human enteroviruses"],"prefix":"10.1002","volume":"82","author":[{"given":"David","family":"Perera","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroyuki","family":"Shimizu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiromu","family":"Yoshida","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Phan","family":"Van 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