{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,16]],"date-time":"2026-01-16T17:20:16Z","timestamp":1768584016992,"version":"3.49.0"},"reference-count":40,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2010,3,24]],"date-time":"2010-03-24T00:00:00Z","timestamp":1269388800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2010,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The live replication\u2010competent modified vaccinia virus Tiantan (MVTT) is an attractive vaccine vector, yet little is known about its tissue tropism and pathology in vivo. Recently, we demonstrated that a recombinant MVTT expressing the spike glycoprotein of SARS\u2010CoV (namely MVTT\u2010S) is superior to the non\u2010replicating modified vaccinia Ankara (MVA\u2010S) for inducing high level of neutralizing antibodies through mucosal vaccination. In this study, we further determined the tissue tropism and safety of MVTT\u2010S after the vaccine was administrated through various routes including: intramuscular (i.m.), intranasal (i.n.), and intravaginal (i.vag.) inoculations, respectively. Using real\u2010time PCR, nested PCR, immunohistochemistry and in situ hybridization assays, we found that MVTT\u2010S was able to produce a transient infection in all cases within 48\u2009hr post\u2010inoculation, yet the major site of viral replication in various tissues or organs was dependent on the route of viral administration. We demonstrated that i.m. injection of MVTT\u2010S primarily targeted draining inguinal lymph nodes, whereas mucosal inoculation had broader range of tissue infections. i.n. inoculation involved infections in lungs, kidneys, spleens and cervix lymph nodes while i.vag. administration targeted uteruses, ovaries, kidneys and spleens. Critically, the infection did not cause severe pathogenic consequences in infected tissues, which was consistent to the attenuated phenotype of MVTT\u2010S. Our findings have implications for the optimization of vaccination route and for studies on the correlation between the magnitude of immune responses and the extent of tissue involvement in vivo. J. Med. Virol. 82: 727\u2013734, 2010. \u00a9 2010 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jmv.21667","type":"journal-article","created":{"date-parts":[[2010,3,25]],"date-time":"2010-03-25T04:23:22Z","timestamp":1269491002000},"page":"727-734","source":"Crossref","is-referenced-by-count":14,"title":["The route of inoculation determines the tissue tropism of modified vaccinia tiantan expressing the spike glycoprotein of SARS\u2010CoV in mice"],"prefix":"10.1002","volume":"82","author":[{"given":"Huan","family":"Liu","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wenbo","family":"Yu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xian","family":"Tang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Haibo","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wenjie","family":"Ouyang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jingying","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhiwei","family":"Chen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2010,3,24]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0401939101"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1097\/COH.0b013e3282f9ae9c"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2796.2008.02053.x"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.71.4.3031-3038.1997"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.79.5.2678-2688.2005"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.70.6.3781-3787.1996"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2003.10.010"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0264-410X(99)00278-9"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1080\/08830180802684331"},{"key":"e_1_2_1_11_1","doi-asserted-by":"crossref","first-page":"1603","DOI":"10.4049\/jimmunol.162.3.1603","article-title":"Mucosal DNA vaccine immunization against measles with a highly attenuated Shigella flexneri vector","volume":"162","author":"Fennelly GJ","year":"1999","journal-title":"J Immunol"},{"key":"e_1_2_1_12_1","doi-asserted-by":"crossref","first-page":"1523","DOI":"10.1089\/aid.1992.8.1523","article-title":"Mucosal approaches to HIV vaccine development","volume":"8","author":"Forrest BD","year":"1992","journal-title":"AIDS Res Hum Retroviruses"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.172.10.6209"},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2008.04.051"},{"key":"e_1_2_1_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2008.03.036"},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm1213"},{"key":"e_1_2_1_17_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0004180"},{"key":"e_1_2_1_18_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.0803871"},{"key":"e_1_2_1_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2005.05.032"},{"key":"e_1_2_1_20_1","doi-asserted-by":"publisher","DOI":"10.1038\/353709c0"},{"key":"e_1_2_1_21_1","volume-title":"Mucosal immunology","author":"Mestecky J","year":"2005"},{"key":"e_1_2_1_22_1","first-page":"129","article-title":"Mucosal immunity, HIV transmission, and AIDS","volume":"68","author":"Miller CJ","year":"1993","journal-title":"Lab Invest"},{"key":"e_1_2_1_23_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-59745-170-3_22"},{"key":"e_1_2_1_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/0140-6736(91)92958-5"},{"key":"e_1_2_1_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2004.05.015"},{"key":"e_1_2_1_26_1","doi-asserted-by":"publisher","DOI":"10.1080\/10611860410001670062"},{"key":"e_1_2_1_27_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.167.1.407"},{"key":"e_1_2_1_28_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00705-003-0006-z"},{"key":"e_1_2_1_29_1","doi-asserted-by":"publisher","DOI":"10.1099\/vir.0.19050-0"},{"key":"e_1_2_1_30_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.163.2.436"},{"key":"e_1_2_1_31_1","doi-asserted-by":"publisher","DOI":"10.1097\/COH.0b013e32830ab9ee"},{"key":"e_1_2_1_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0264-410X(01)00330-9"},{"key":"e_1_2_1_33_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pmed.0050081"},{"key":"e_1_2_1_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0264-410X(02)00118-4"},{"key":"e_1_2_1_35_1","doi-asserted-by":"publisher","DOI":"10.1089\/vim.2007.0103"},{"key":"e_1_2_1_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/0264-410X(94)90341-7"},{"key":"e_1_2_1_37_1","doi-asserted-by":"publisher","DOI":"10.1089\/0889222041725253"},{"key":"e_1_2_1_38_1","doi-asserted-by":"publisher","DOI":"10.1097\/COH.0b013e32830634c6"},{"key":"e_1_2_1_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0264-410X(99)00257-1"},{"key":"e_1_2_1_40_1","doi-asserted-by":"publisher","DOI":"10.1038\/gt.2008.181"},{"key":"e_1_2_1_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jviromet.2007.03.012"}],"container-title":["Journal of Medical Virology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjmv.21667","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jmv.21667","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,22]],"date-time":"2023-11-22T14:29:18Z","timestamp":1700663358000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jmv.21667"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,3,24]]},"references-count":40,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2010,5]]}},"alternative-id":["10.1002\/jmv.21667"],"URL":"https:\/\/doi.org\/10.1002\/jmv.21667","archive":["Portico"],"relation":{},"ISSN":["0146-6615","1096-9071"],"issn-type":[{"value":"0146-6615","type":"print"},{"value":"1096-9071","type":"electronic"}],"subject":[],"published":{"date-parts":[[2010,3,24]]}}}