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A near patient test (NPT) for the detection of mumps\u2010specific IgM in oral fluid specimens was developed and evaluated using 196 oral fluid specimens from suspected cases of mumps and measles. Compared to enzyme immunoassay (EIA), the sensitivity, specificity, positive and negative predictive value of the mumps IgM NPT were 79.5%, 100%, 100%, and 72.6%, respectively. On oral fluid specimens with a test to negative control optical density ratio (T\/N) \u226510 in the EIA, sensitivity of the NPT increased to 95.2%. To determine whether viral nucleic acid from oral fluid was preserved on the NPT strips used for IgM detection, reverse transcription\u2010polymerase chain reaction (RT\u2010PCR) was performed on 58 oral fluids before and after testing on NPT strips. In RT\u2010PCR\u2010positive oral fluids, mumps virus was detected in 19\/21 (90.5%) specimens extracted from NPT strips. Mumps virus RNA was not detected in 37\/37 (100%) NPT strips from mumps RT\u2010PCR\u2010negative oral fluid specimens. Mumps IgM NPT is rapid and simple to perform, with an acceptable level of performance for confirmation of a clinical diagnosis outside of the laboratory. The NPT strip is also a suitable matrix for preserving nucleic acid, enabling virus\u2010specific RT\u2010PCR to be performed. J. Med. Virol. 82:485\u2013493, 2010. \u00a9 2010 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jmv.21693","type":"journal-article","created":{"date-parts":[[2010,1,19]],"date-time":"2010-01-19T19:51:41Z","timestamp":1263930701000},"page":"485-493","source":"Crossref","is-referenced-by-count":10,"title":["Development and evaluation of a rapid immunochromatographic test for mumps\u2010specific IgM in oral fluid specimens and use as a matrix for preserving viral nucleic acid for 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