{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T06:32:45Z","timestamp":1791181965102,"version":"4.1.0"},"reference-count":32,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2010,1,19]],"date-time":"2010-01-19T00:00:00Z","timestamp":1263859200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2010,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Merkel cell polyomavirus (MCPyV) has been found to be integrated monoclonally in a rare skin cancer named Merkel cell carcinoma. More recently, MCPyV has been detected in the upper respiratory tract of pediatric and adult patients. However, the mode of transmission and pathogenic role of MCPyV in the respiratory system has not been determined. In this study, MCPyV was sought in the lower respiratory tract of adult patients admitted to the hospital. MCPyV DNA was detected in 15 (17.24%) out of 87 lower respiratory tract samples. Most of the patients with MCPyV were over 50 years old. Nucleotide sequence of the t\u2010antigen of MCPyV identified in respiratory secretions showed a homology to those found in Merkel cell carcinoma. In addition, phylogenetic analysis undertaken on the t\u2010antigen sequences of Italian isolates and other MCPyVs identified in healthy and cancer tissues showed that all these isolates belonged to the same clade. Selective pressure analysis for the t\u2010antigen revealed the presence of five sites under positive selection (\u03c9\u2009=\u20094.3), with a posterior probabilities above 0.99. The \u03b1 parameter of the gamma distribution was 0.01, showing that this distribution has a characteristic L\u2010shape and suggesting a strong nucleotide substitution rate heterogeneity across sites. This study shows that MCPyV can infect the lower respiratory tract, but further investigations are needed to define its pathogenic role in respiratory diseases. J. Med. Virol. 82:505\u2013509, 2010. \u00a9 2010 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jmv.21711","type":"journal-article","created":{"date-parts":[[2010,1,19]],"date-time":"2010-01-19T19:55:18Z","timestamp":1263930918000},"page":"505-509","source":"Crossref","is-referenced-by-count":49,"title":["Identification of Merkel cell polyomavirus in the lower respiratory tract of Italian patients"],"prefix":"10.1002","volume":"82","author":[{"given":"Muhammed","family":"Babakir\u2010Mina","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Massimo","family":"Ciccozzi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alessandra","family":"Lo Presti","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francesco","family":"Greco","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carlo Federico","family":"Perno","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marco","family":"Ciotti","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2010,1,19]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.21303"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.21417"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.21559"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/jid.2008.198"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcv.2007.11.001"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.3201\/eid1503.081067"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcv.2008.11.008"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/jid.2008.302"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.50.2.541-546.1984"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1152586"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.3201\/eid1409.080651"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/jid.2008.229"},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01635-08"},{"key":"e_1_2_1_15_1","doi-asserted-by":"publisher","DOI":"10.3201\/eid1503.081206"},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00299-001-0399-7"},{"key":"e_1_2_1_17_1","first-page":"2268","volume-title":"Fields virology","author":"Imperiale MJ","year":"2007"},{"key":"e_1_2_1_18_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-08-0949"},{"key":"e_1_2_1_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.24323"},{"key":"e_1_2_1_20_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1000363"},{"key":"e_1_2_1_21_1","doi-asserted-by":"publisher","DOI":"10.1093\/genetics\/148.3.929"},{"key":"e_1_2_1_22_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1095-8312.2004.00368.x"},{"key":"e_1_2_1_23_1","doi-asserted-by":"publisher","DOI":"10.1086\/321813"},{"key":"e_1_2_1_24_1","doi-asserted-by":"publisher","DOI":"10.1038\/jid.2008.215"},{"key":"e_1_2_1_25_1","doi-asserted-by":"publisher","DOI":"10.1002\/path.2532"},{"key":"e_1_2_1_26_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0806526105"},{"key":"e_1_2_1_27_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.24510"},{"key":"e_1_2_1_28_1","first-page":"160","volume-title":"The phylogenetic handbook\u2014A practical approach to DNA and protein phylogeny","author":"Swofford D","year":"2003"},{"key":"e_1_2_1_29_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/25.24.4876"},{"key":"e_1_2_1_30_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0004958"},{"key":"e_1_2_1_31_1","first-page":"555","article-title":"PAML: A program package for phylogenetic analysis by maximum likelihood","volume":"13","author":"Yang Z","year":"1997","journal-title":"Comput Appl Biosci"},{"key":"e_1_2_1_32_1","doi-asserted-by":"publisher","DOI":"10.1093\/genetics\/155.1.431"},{"key":"e_1_2_1_33_1","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/msi097"}],"container-title":["Journal of Medical Virology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjmv.21711","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jmv.21711","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,22]],"date-time":"2023-11-22T15:36:50Z","timestamp":1700667410000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jmv.21711"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,1,19]]},"references-count":32,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2010,3]]}},"alternative-id":["10.1002\/jmv.21711"],"URL":"https:\/\/doi.org\/10.1002\/jmv.21711","archive":["Portico"],"relation":{},"ISSN":["0146-6615","1096-9071"],"issn-type":[{"value":"0146-6615","type":"print"},{"value":"1096-9071","type":"electronic"}],"subject":[],"published":{"date-parts":[[2010,1,19]]}}}