{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,11,23]],"date-time":"2023-11-23T00:04:17Z","timestamp":1700697857432},"reference-count":40,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2010,1,19]],"date-time":"2010-01-19T00:00:00Z","timestamp":1263859200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2010,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Human enteroviruses (EVs) cause a broad spectrum of acute and chronic diseases including meningitis and myocarditis. The type I interferon\u2010induced MxA protein has been shown to inhibit the replication of an EV, coxsackievirus B4 (CVB4), but not cardioviruses such as encephalomyocarditis virus and mengo virus, members of the Picornaviridae family. EVs consist of more than 60 distinct serotypes against which the antiviral activity of MxA was not investigated yet. The main aim of this study was to explore the antiviral activity of MxA protein against a clinical CVB1 isolate and other EV prototypes. Vero cells expressing constituvely MxA protein were infected with EVs, and the percentage of inhibiton of expression of enteroviral RNA and capsid VP1 protein was determined. Following infection of MxA\u2010transfected Vero cells with EVs, the expression of enteroviral RNA was inhibited by up to 99%, and that of VP1 protein by up to 85%. However, there was a difference in the percentage of MxA inhibition of EV replication between the different EV prototypes. This difference in MxA sensitivity was not due to a difference in the viral replication rates. The MxA protein was inactive against the clinical CVB1 isolate, and the replication rate of CVB1 isolate in MxA\u2010transfected Vero cells was higher than that in mock\u2010transfected Vero cells. A serial passage of the clinical CVB1 isolate and other EV prototypes resulted in an increase in their susceptibility to MxA protein. These results suggest the presence of MxA\u2010resistant EV variants that may escape innate immunity and cause disease. J. Med. Virol. 82:424\u2013432, 2010. \u00a9 2010 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jmv.21713","type":"journal-article","created":{"date-parts":[[2010,1,19]],"date-time":"2010-01-19T19:55:44Z","timestamp":1263930944000},"page":"424-432","source":"Crossref","is-referenced-by-count":3,"title":["Difference in susceptibility to MxA protein between a coxsackievirus B1 isolate and prototype, impact of serial cell culture passage"],"prefix":"10.1002","volume":"82","author":[{"given":"Wassim","family":"Chehadeh","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hayfaa A.","family":"Abdulkareem","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2010,1,19]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1177\/014107688808100608"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-642-73610-0_33"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/175.3.697"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00313-08"},{"key":"e_1_2_1_6_1","first-page":"74","volume-title":"Virology, a laboratory manual","author":"Burleson FG","year":"1992"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1086\/315516"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0264-410X(98)00079-6"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1006\/viro.2001.0877"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(95)91270-3"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcv.2008.10.007"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.85.14.5259"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1159\/000263911"},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-5822.2007.00964.x"},{"key":"e_1_2_1_15_1","doi-asserted-by":"crossref","first-page":"3904","DOI":"10.1128\/jvi.69.6.3904-3909.1995","article-title":"Human MxA protein inhibits tick\u2010borne Thogoto virus but not Dhori virus","volume":"69","author":"Frese M","year":"1995","journal-title":"J Virol"},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.1086\/314758"},{"key":"e_1_2_1_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.micinf.2007.09.010"},{"key":"e_1_2_1_18_1","doi-asserted-by":"publisher","DOI":"10.1007\/s000590050022"},{"key":"e_1_2_1_19_1","doi-asserted-by":"publisher","DOI":"10.1159\/000081735"},{"key":"e_1_2_1_20_1","doi-asserted-by":"publisher","DOI":"10.1089\/jir.1985.5.289"},{"key":"e_1_2_1_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/nri1703"},{"key":"e_1_2_1_22_1","first-page":"174","volume-title":"Human MxA protein in transfected Vero cells: No inhibition of paramyxoviruses","author":"Kopp U","year":"1993"},{"key":"e_1_2_1_23_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.72.2.1516-1522.1998"},{"key":"e_1_2_1_24_1","doi-asserted-by":"publisher","DOI":"10.1101\/gr.20102"},{"key":"e_1_2_1_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.virusres.2007.05.006"},{"key":"e_1_2_1_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0264-410X(02)00182-2"},{"key":"e_1_2_1_27_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.21374"},{"key":"e_1_2_1_28_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.43.9.2109"},{"key":"e_1_2_1_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/0732-8893(84)90069-5"},{"key":"e_1_2_1_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0166-3542(01)00206-6"},{"key":"e_1_2_1_31_1","doi-asserted-by":"publisher","DOI":"10.1097\/00006454-199912000-00002"},{"key":"e_1_2_1_32_1","doi-asserted-by":"publisher","DOI":"10.1006\/viro.2000.0485"},{"key":"e_1_2_1_33_1","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dki263"},{"key":"e_1_2_1_34_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.20933"},{"key":"e_1_2_1_35_1","doi-asserted-by":"crossref","first-page":"6910","DOI":"10.1128\/jvi.68.11.6910-6917.1994","article-title":"Cell type\u2010specific MxA\u2010mediated inhibition of measles virus transcription in human brain cells","volume":"68","author":"Schneider\u2010Schaulies S","year":"1994","journal-title":"J Virol"},{"key":"e_1_2_1_36_1","first-page":"1","article-title":"Interferon\u2010induced Mx protein: A mediator of cellular resistance to influenza virus","volume":"8","author":"Staeheli P","year":"1987","journal-title":"Interferon"},{"key":"e_1_2_1_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/0962-8924(93)90055-6"},{"key":"e_1_2_1_38_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.biochem.67.1.227"},{"key":"e_1_2_1_39_1","doi-asserted-by":"publisher","DOI":"10.1006\/viro.1995.1268"},{"key":"e_1_2_1_40_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.39.11.4093-4096.2001"},{"key":"e_1_2_1_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1386-6532(02)00032-X"}],"container-title":["Journal of Medical Virology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjmv.21713","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jmv.21713","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,22]],"date-time":"2023-11-22T15:35:55Z","timestamp":1700667355000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jmv.21713"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,1,19]]},"references-count":40,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2010,3]]}},"alternative-id":["10.1002\/jmv.21713"],"URL":"https:\/\/doi.org\/10.1002\/jmv.21713","archive":["Portico"],"relation":{},"ISSN":["0146-6615","1096-9071"],"issn-type":[{"value":"0146-6615","type":"print"},{"value":"1096-9071","type":"electronic"}],"subject":[],"published":{"date-parts":[[2010,1,19]]}}}